Eruca sativa seed napin structural insights and thorough functional characterization.
2S Albumins, Plant
/ chemistry
Amino Acid Sequence
Antifungal Agents
/ chemistry
Brassica
/ chemistry
Chromatography, Liquid
Isoelectric Focusing
Microbial Sensitivity Tests
Models, Molecular
Plant Proteins
/ chemistry
Protein Conformation
Scattering, Small Angle
Seeds
/ chemistry
Structure-Activity Relationship
Tandem Mass Spectrometry
X-Ray Diffraction
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 12 2021
15 12 2021
Historique:
received:
09
08
2021
accepted:
01
11
2021
entrez:
16
12
2021
pubmed:
17
12
2021
medline:
27
1
2022
Statut:
epublish
Résumé
A potent napin protein has been thoroughly characterized from seeds of rocket salad (Eruca sativa). Eruca sativa napin (EsNap) was purified by ammonium sulfate precipitation (70%) and size-exclusion chromatography. Single intact 16 kDa EsNap band was reduced to 11 and 5 kDa bands respectively on SDS-PAGE. Nano LC-MS/MS yielded two fragments comprising of 26 residues which showed 100% sequence identity with napin-3 of Brassica napus. CD spectroscopy indicated a dominant α-helical structure of EsNap. Monodispersity of EsNap was verified by dynamic light scattering, which also confirmed the monomeric status with a corresponding hydrodynamic radius of 2.4 ± 0.2 nm. An elongated ab initio shape of EsNap was calculated based on SAXS data, with an R
Identifiants
pubmed: 34911985
doi: 10.1038/s41598-021-02174-6
pii: 10.1038/s41598-021-02174-6
pmc: PMC8674280
doi:
Substances chimiques
2S Albumins, Plant
0
Antifungal Agents
0
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
24066Informations de copyright
© 2021. The Author(s).
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