Study of plasmid-based expression level heterogeneity under plasmid-curing like conditions in Cupriavidus necator.

Biosensing Techniques Cupriavidus necator / genetics Kinetics Plasmids / genetics
Chemostat Expression level Flow cytometry Plasmid curing Plasmid stability Single-cell analysis

Journal

Journal of biotechnology
ISSN: 1873-4863
Titre abrégé: J Biotechnol
Pays: Netherlands
ID NLM: 8411927

Informations de publication

Date de publication:
10 Feb 2022
Historique:
received: 29 07 2021
revised: 24 12 2021
accepted: 30 12 2021
pubmed: 8 1 2022
medline: 2 2 2022
entrez: 7 1 2022
Statut: ppublish

Résumé

Plasmid expression level heterogeneity in Cupriavidus necator was studied in response to stringent culture conditions, supposed to enhance plasmid instability, through plasmid curing strategies. Two plasmid curing strategies were compared based on their efficiency at generating heterogeneity in batch: rifampicin addition and temperature increase. A temperature increase from 30° to 37 °C was the most efficient plasmid curing strategy. To generate a heterogeneous population in terms of plasmid expression levels, successive batches at supra-optimal culture temperature (i.e. 37 °C) were initially conducted. Three distinct fluorescent subpopulations P

Identifiants

DOI: 10.1016/j.jbiotec.2021.12.015 PMID: 34995560
pubmed: 34995560
pii: S0168-1656(22)00003-7
doi: 10.1016/j.jbiotec.2021.12.015
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

17-29

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Catherine Boy (C)

TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.

Julie Lesage (J)

TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.

Sandrine Alfenore (S)

TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.

Stéphane E Guillouet (SE)

TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France.

Nathalie Gorret (N)

TBI, Université de Toulouse, CNRS, INRA, INSA, Toulouse, France. Electronic address: ngorret@insa-toulouse.fr.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Study of plasmid-based expression level...
questionsmedicales.fr › Bactéries › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus › Cupriavidus necator › Study of plasmid-based expression level...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Cinétique › Study of plasmid-based expression level...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Plasmides › Study of plasmid-based expression level...