Cap analysis of gene expression reveals alternative promoter usage in a rat model of hypertension.
Journal
Life science alliance
ISSN: 2575-1077
Titre abrégé: Life Sci Alliance
Pays: United States
ID NLM: 101728869
Informations de publication
Date de publication:
04 2022
04 2022
Historique:
received:
15
09
2021
revised:
26
12
2021
accepted:
28
12
2021
entrez:
8
1
2022
pubmed:
9
1
2022
medline:
23
3
2022
Statut:
epublish
Résumé
The role of alternative promoter usage in tissue-specific gene expression has been well established; however, its role in complex diseases is poorly understood. We performed cap analysis of gene expression (CAGE) sequencing from the left ventricle of a rat model of hypertension, the spontaneously hypertensive rat (SHR), and a normotensive strain, Brown Norway to understand the role of alternative promoter usage in complex disease. We identified 26,560 CAGE-defined transcription start sites in the rat left ventricle, including 1,970 novel cardiac transcription start sites. We identified 28 genes with alternative promoter usage between SHR and Brown Norway, which could lead to protein isoforms differing at the amino terminus between two strains and 475 promoter switching events altering the length of the 5' UTR. We found that the shift in
Identifiants
pubmed: 34996843
pii: 5/4/e202101234
doi: 10.26508/lsa.202101234
pmc: PMC8742872
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© 2022 Dahale et al.
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