Identification of sequence-specific promoters driving polycistronic transcription initiation by RNA polymerase II in trypanosomes.
African trypanosomes
ChIP-seq
RNA polymerase II
core promoter
gene expression
nucleotide motifs
polycistronic transcription
transcription initiation site
transcriptional activity
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
11 01 2022
11 01 2022
Historique:
received:
12
03
2021
revised:
18
10
2021
accepted:
15
12
2021
entrez:
12
1
2022
pubmed:
13
1
2022
medline:
15
2
2022
Statut:
ppublish
Résumé
Protein-coding genes in trypanosomes occur in polycistronic transcription units (PTUs). How RNA polymerase II (Pol II) initiates transcription of PTUs has not been resolved; the current model favors chromatin modifications inducing transcription rather than sequence-specific promoters. Here, we uncover core promoters by functional characterization of Pol II peaks identified by chromatin immunoprecipitation sequencing (ChIP-seq). Two distinct promoters are located between divergent PTUs, each driving unidirectional transcription. Detailed analysis identifies a 75-bp promoter that is necessary and sufficient to drive full reporter expression and contains functional motifs. Analysis of further promoters suggests transcription initiation is regulated and promoters are either focused or dispersed. In contrast to the previous model of unregulated and promoter-independent transcription initiation, we find that sequence-specific promoters determine the initiation of Pol II transcription of protein-coding genes PTUs. These findings in Trypanosoma brucei suggest that in addition of chromatin modifications, promoter motifs-based regulation of gene expression is deeply conserved among eukaryotes.
Identifiants
pubmed: 35021094
pii: S2211-1247(21)01725-3
doi: 10.1016/j.celrep.2021.110221
pii:
doi:
Substances chimiques
Protozoan Proteins
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
110221Subventions
Organisme : NIAID NIH HHS
ID : R01 AI114685
Pays : United States
Organisme : Wellcome
ID : 217138/Z/19/Z
Informations de copyright
Copyright © 2021 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.