Pseudouridine synthase 7 is an opportunistic enzyme that binds and modifies substrates with diverse sequences and structures.
Amino Acid Sequence
Binding Sites
Catalytic Domain
Models, Molecular
Protein Binding
Protein Conformation
Protein Interaction Domains and Motifs
RNA, Fungal
/ chemistry
RNA, Messenger
/ chemistry
Saccharomyces cerevisiae Proteins
/ chemistry
Stress, Physiological
Structure-Activity Relationship
Substrate Specificity
Temperature
Thermodynamics
Pus7
RNA modification
TruD
pseudouridine
structure
Journal
Proceedings of the National Academy of Sciences of the United States of America
ISSN: 1091-6490
Titre abrégé: Proc Natl Acad Sci U S A
Pays: United States
ID NLM: 7505876
Informations de publication
Date de publication:
25 01 2022
25 01 2022
Historique:
accepted:
17
11
2021
entrez:
21
1
2022
pubmed:
22
1
2022
medline:
1
3
2022
Statut:
ppublish
Résumé
Pseudouridine (Ψ) is a ubiquitous RNA modification incorporated by pseudouridine synthase (Pus) enzymes into hundreds of noncoding and protein-coding RNA substrates. Here, we determined the contributions of substrate structure and protein sequence to binding and catalysis by pseudouridine synthase 7 (Pus7), one of the principal messenger RNA (mRNA) modifying enzymes. Pus7 is distinct among the eukaryotic Pus proteins because it modifies a wider variety of substrates and shares limited homology with other Pus family members. We solved the crystal structure of
Identifiants
pubmed: 35058356
pii: 2109708119
doi: 10.1073/pnas.2109708119
pmc: PMC8794802
pii:
doi:
Substances chimiques
Pus7 protein, S cerevisiae
0
RNA, Fungal
0
RNA, Messenger
0
Saccharomyces cerevisiae Proteins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NIGMS NIH HHS
ID : T32 GM132046
Pays : United States
Informations de copyright
Copyright © 2022 the Author(s). Published by PNAS.
Déclaration de conflit d'intérêts
The authors declare no competing interest.
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