Chromatin Immunoprecipitation in Chloroplasts.
chloroplast
chromatin immunoprecipitation
genome
Journal
Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894
Informations de publication
Date de publication:
Jan 2022
Jan 2022
Historique:
entrez:
25
1
2022
pubmed:
26
1
2022
medline:
28
1
2022
Statut:
ppublish
Résumé
Chromatin is the genetic material assembled by nucleic acids (including DNA and RNA) and proteins. The biological functions of chromatin are highly dependent on the interaction between DNA (and/or RNA) and proteins that bind to it. Chromatin immunoprecipitation (ChIP) is a powerful technique for evaluating these interactions and has been widely used to characterize the functions of nuclear proteins. However, its application in identifying plant organellar chromatin-binding proteins is lagging. This article describes the method for analyzing the association of chloroplast-localized proteins with the chloroplast genome. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Chloroplast isolation Basic Protocol 2: Crosslinking of DNA-Protein complexes Basic Protocol 3: Chromatin isolation and preparation Support Protocol: Bead-antibody complex preparation Basic Protocol 4: Immunoprecipitation and washes Basic Protocol 5: DNA preparation Basic Protocol 6: Analysis of results.
Substances chimiques
Chromatin
0
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e360Subventions
Organisme : National Natural Science Foundation of China
ID : 31822028
Organisme : National Natural Science Foundation of China
ID : 32170321
Organisme : National Natural Science Foundation of China
ID : 91940306
Organisme : Ministry of Science and Technology of China
ID : 2016YFA0500800
Informations de copyright
© 2022 Wiley Periodicals LLC.
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