Chromatin Immunoprecipitation in Chloroplasts.


Journal

Current protocols
ISSN: 2691-1299
Titre abrégé: Curr Protoc
Pays: United States
ID NLM: 101773894

Informations de publication

Date de publication:
Jan 2022
Historique:
entrez: 25 1 2022
pubmed: 26 1 2022
medline: 28 1 2022
Statut: ppublish

Résumé

Chromatin is the genetic material assembled by nucleic acids (including DNA and RNA) and proteins. The biological functions of chromatin are highly dependent on the interaction between DNA (and/or RNA) and proteins that bind to it. Chromatin immunoprecipitation (ChIP) is a powerful technique for evaluating these interactions and has been widely used to characterize the functions of nuclear proteins. However, its application in identifying plant organellar chromatin-binding proteins is lagging. This article describes the method for analyzing the association of chloroplast-localized proteins with the chloroplast genome. © 2022 Wiley Periodicals LLC. Basic Protocol 1: Chloroplast isolation Basic Protocol 2: Crosslinking of DNA-Protein complexes Basic Protocol 3: Chromatin isolation and preparation Support Protocol: Bead-antibody complex preparation Basic Protocol 4: Immunoprecipitation and washes Basic Protocol 5: DNA preparation Basic Protocol 6: Analysis of results.

Identifiants

pubmed: 35077029
doi: 10.1002/cpz1.360
doi:

Substances chimiques

Chromatin 0
DNA 9007-49-2

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e360

Subventions

Organisme : National Natural Science Foundation of China
ID : 31822028
Organisme : National Natural Science Foundation of China
ID : 32170321
Organisme : National Natural Science Foundation of China
ID : 91940306
Organisme : Ministry of Science and Technology of China
ID : 2016YFA0500800

Informations de copyright

© 2022 Wiley Periodicals LLC.

Références

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Auteurs

Wenjie Wang (W)

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Tsinghua-Peking Center for Life Sciences, Beijing, China.

Lingling Cheng (L)

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.

Qianwen Sun (Q)

Center for Plant Biology, School of Life Sciences, Tsinghua University, Beijing, China.
Tsinghua-Peking Center for Life Sciences, Beijing, China.

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Classifications MeSH