A Chimeric DNA/RNA Antiparallel Quadruplex with Improved Stability.

DNA RNA quadruplex stability thrombin-binding aptamer

Journal

ChemistryOpen
ISSN: 2191-1363
Titre abrégé: ChemistryOpen
Pays: Germany
ID NLM: 101594811

Informations de publication

Date de publication:
02 2022
Historique:
revised: 13 12 2021
received: 25 11 2021
entrez: 1 2 2022
pubmed: 2 2 2022
medline: 26 4 2022
Statut: ppublish

Résumé

Nucleic acid quadruplexes are proposed to play a role in the regulation of gene expression, are often present in aptamers selected for specific binding functions and have potential applications in medicine and biotechnology. Therefore, understanding their structure and thermodynamic properties and designing highly stable quadruplexes is desirable for a variety of applications. Here, we evaluate DNA→RNA substitutions in the context of a monomolecular, antiparallel quadruplex, the thrombin-binding aptamer (TBA, GGTTGGTGTGGTTGG) in the presence of either K

Identifiants

pubmed: 35103415
doi: 10.1002/open.202100276
pmc: PMC8805387
doi:

Substances chimiques

RNA 63231-63-0
DNA 9007-49-2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e202100276

Subventions

Organisme : Shota Rustaveli National Science Foundation
ID : FR17_140
Organisme : Dan Schoenberg RNA Undergraduate Research Fellowship

Informations de copyright

© 2022 The Authors. Published by Wiley-VCH GmbH.

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Auteurs

Elaina P Boyle (EP)

Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.

Levan Lomidze (L)

Institute of Biophysics, Ilia State University, Tbilisi, 0162, Republic of Georgia.

Karin Musier-Forsyth (K)

Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.

Besik Kankia (B)

Department of Chemistry and Biochemistry, The Ohio State University, Columbus, OH 43210, USA.
Center for RNA Biology, The Ohio State University, Columbus, OH 43210, USA.
Institute of Biophysics, Ilia State University, Tbilisi, 0162, Republic of Georgia.

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Classifications MeSH