Tandem affinity purification protocol for isolation of protein complexes from

Mass Spectrometry Proteins / metabolism Schizosaccharomyces / genetics Tandem Affinity Purification
Cell Biology Model Organisms Protein Biochemistry Protein expression and purification

Journal

STAR protocols
ISSN: 2666-1667
Titre abrégé: STAR Protoc
Pays: United States
ID NLM: 101769501

Informations de publication

Date de publication:
18 03 2022
Historique:
entrez: 7 2 2022
pubmed: 8 2 2022
medline: 9 4 2022
Statut: epublish

Résumé

Many cellular processes require the activities of complex molecular machines composed of several protein subunits. Insights into these systems can be gained by isolation of protein complexes followed by

Identifiants

DOI: 10.1016/j.xpro.2022.101137 PMID: 35128479 PMC: PMC8808283
pubmed: 35128479
doi: 10.1016/j.xpro.2022.101137
pii: S2666-1667(22)00017-X
pmc: PMC8808283
doi:

Substances chimiques

Proteins 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

101137

Subventions

Organisme : Austrian Science Fund FWF
ID : P 30516
Pays : Austria

Informations de copyright

© 2022 The Authors.

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Cell Cycle. 2013 Mar 15;12(6):884-8
pubmed: 23462181
Methods Mol Biol. 2014;1177:121-33
pubmed: 24943319
Genes (Basel). 2021 Jun 30;12(7):
pubmed: 34208949
Methods. 2004 Jul;33(3):239-44
pubmed: 15157891
Proteomics. 2009 Oct;9(20):4825-8
pubmed: 19750511
Electrophoresis. 1988 Jun;9(6):288-91
pubmed: 2466660
PLoS Genet. 2015 May 20;11(5):e1005225
pubmed: 25993311
Int J Mol Sci. 2021 Jun 29;22(13):
pubmed: 34209806
Cell Cycle. 2010 Oct 1;9(19):3997-4004
pubmed: 20935472
Nucleic Acids Res. 2016 Feb 29;44(4):1703-17
pubmed: 26682798
J Cell Sci. 2018 Jul 6;131(13):
pubmed: 29898918
Methods Enzymol. 2010;470:759-95
pubmed: 20946835
Nat Biotechnol. 1999 Oct;17(10):1030-2
pubmed: 10504710
Cell Cycle. 2020 Jul;19(14):1777-1785
pubmed: 32594847
Nat Protoc. 2014 Jan;9(1):223-31
pubmed: 24385151
Cold Spring Harb Protoc. 2017 Mar 1;2017(3):
pubmed: 28250214
Methods Enzymol. 2015;559:37-52
pubmed: 26096502
Cell Cycle. 2019 Jul;18(14):1532-1536
pubmed: 31219728

Auteurs

Lubos Cipak (L)

Department of Genetics, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Tomas Selicky (T)

Department of Genetics, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Jan Jurcik (J)

Department of Genetics, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Ingrid Cipakova (I)

Department of Genetics, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Michaela Osadska (M)

Department of Genetics, Cancer Research Institute, Biomedical Research Center of the Slovak Academy of Sciences, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Veronika Lukacova (V)

Medirex Group Academy, Novozamocka 67, 949 05 Nitra, Slovakia.

Peter Barath (P)

Medirex Group Academy, Novozamocka 67, 949 05 Nitra, Slovakia.
Department of Glycobiology, Slovak Academy of Sciences, Institute of Chemistry, Dubravska Cesta 9, 845 05 Bratislava, Slovakia.

Juraj Gregan (J)

Department of Applied Genetics and Cell Biology, Institute of Microbial Genetics, University of Natural Resources and Life Sciences, Vienna (BOKU), Konrad Lorenz Strasse 24, 3430 Tulln an der Donau, Austria.

Articles similaires

Structural duality enables a single protein to act as a toxin-antidote pair for meiotic drive.

Yu Hua, Jianxiu Zhang, Man-Yun Yang et al.
1.00
Meiosis Schizosaccharomyces Schizosaccharomyces pombe Proteins Spores, Fungal

Exploring structural diversity across the protein universe with The Encyclopedia of Domains.

Andy M Lau, Nicola Bordin, Shaun M Kandathil et al.
1.00
Databases, Protein Protein Domains Protein Folding Proteins Deep Learning

A meiotic driver hijacks an epigenetic reader to disrupt mitosis in noncarrier offspring.

Yu Hua, Jianxiu Zhang, Man-Yun Yang et al.
1.00
Schizosaccharomyces Meiosis Schizosaccharomyces pombe Proteins Mitosis Epigenesis, Genetic

Reflection mode polarimetry guides laser mass spectrometry to diagnostically important regions of human breast cancer tissue.

Michael D Singh, Lan Anna Ye, Michael Woolman et al.
1.00
Humans Breast Neoplasms Female Mass Spectrometry Adipose Tissue

Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Spectrométrie de masse › Tandem affinity purification protocol for...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Tandem affinity purification protocol for...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Schizosaccharomyces › Tandem affinity purification protocol for...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie d'affinité › Purification par affinité en tandem › Tandem affinity purification protocol for...