Xrn1 is a deNADding enzyme modulating mitochondrial NAD-capped RNA.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
16 02 2022
16 02 2022
Historique:
received:
24
05
2021
accepted:
18
01
2022
entrez:
17
2
2022
pubmed:
18
2
2022
medline:
3
3
2022
Statut:
epublish
Résumé
The existence of non-canonical nicotinamide adenine diphosphate (NAD) 5'-end capped RNAs is now well established. Nevertheless, the biological function of this nucleotide metabolite cap remains elusive. Here, we show that the yeast Saccharomyces cerevisiae cytoplasmic 5'-end exoribonuclease Xrn1 is also a NAD cap decapping (deNADding) enzyme that releases intact NAD and subsequently degrades the RNA. The significance of Xrn1 deNADding is evident in a deNADding deficient Xrn1 mutant that predominantly still retains its 5'-monophosphate exonuclease activity. This mutant reveals Xrn1 deNADding is necessary for normal growth on non-fermenting sugar and is involved in modulating mitochondrial NAD-capped RNA levels and may influence intramitochondrial NAD levels. Our findings uncover a contribution of mitochondrial NAD-capped RNAs in overall NAD regulation with the deNADding activity of Xrn1 fulfilling a central role.
Identifiants
pubmed: 35173156
doi: 10.1038/s41467-022-28555-7
pii: 10.1038/s41467-022-28555-7
pmc: PMC8850482
doi:
Substances chimiques
RNA Caps
0
RNA, Messenger
0
RNA, Mitochondrial
0
RNA-Binding Proteins
0
Rai1 protein, S cerevisiae
0
Saccharomyces cerevisiae Proteins
0
NAD
0U46U6E8UK
RAT1 protein, S cerevisiae
147883-15-6
Exoribonucleases
EC 3.1.-
XRN1 protein, S cerevisiae
EC 3.1.11.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
889Subventions
Organisme : NIDCD NIH HHS
ID : R01 DC015000
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM126488
Pays : United States
Informations de copyright
© 2022. The Author(s).
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