Comparative physiological and transcriptomic analysis reveal MdWRKY75 associated with sucrose accumulation in postharvest 'Honeycrisp' apples with bitter pit.
Ascorbic Acid
/ metabolism
Calcium
/ metabolism
Flavonoids
/ metabolism
Food Storage
Fruit
/ genetics
Gene Expression Profiling
Gene Expression Regulation, Plant
Hydrogen Peroxide
/ metabolism
Malus
/ genetics
Phenols
/ metabolism
Plant Proteins
/ genetics
Reactive Oxygen Species
/ metabolism
Seeds
Sucrose
/ metabolism
Transcription Factors
/ genetics
Apple (Malus domestica)
Calcium deficiency
MdSWEET1
MdWRKY75
Sucrose-metabolism
Journal
BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807
Informations de publication
Date de publication:
17 Feb 2022
17 Feb 2022
Historique:
received:
18
10
2021
accepted:
03
02
2022
entrez:
18
2
2022
pubmed:
19
2
2022
medline:
26
2
2022
Statut:
epublish
Résumé
Calcium (Ca) deficiency can cause apple bitter pit, reduce the quality and shelf life. WRKY transcription factors play essential role in plant response to multiple disorders. However, the underlying mechanisms causing bitter pit in apple fruit due to Ca deficiency during storage is extremely limited. In the present study, the nutritional metabolites and reactive oxygen species (ROS) were compared in Ca-deficient and healthy apple fruit (CK) during storage. Results showed that Ca-deficient apples sustained significantly higher production of ROS, PPO activity, flavonoids, total phenol, total soluble solids (TSS), and sucrose contents, but the contents of Ca, H Calcium deficiency could decrease antioxidant capacity, accelerate nutritional metabolism and up-regulate the expression of WRKYs in apple with bitter pit. Overexpression of MdWRKY75 significantly increased sucrose accumulation and the expression of MdSWEET1. These findings further strengthened knowledge of the basic molecular mechanisms in calcium deficiency apple flesh and contributed to improving the nutritional quality of apple fruit.
Sections du résumé
BACKGROUND
BACKGROUND
Calcium (Ca) deficiency can cause apple bitter pit, reduce the quality and shelf life. WRKY transcription factors play essential role in plant response to multiple disorders. However, the underlying mechanisms causing bitter pit in apple fruit due to Ca deficiency during storage is extremely limited.
RESULTS
RESULTS
In the present study, the nutritional metabolites and reactive oxygen species (ROS) were compared in Ca-deficient and healthy apple fruit (CK) during storage. Results showed that Ca-deficient apples sustained significantly higher production of ROS, PPO activity, flavonoids, total phenol, total soluble solids (TSS), and sucrose contents, but the contents of Ca, H
CONCLUSIONS
CONCLUSIONS
Calcium deficiency could decrease antioxidant capacity, accelerate nutritional metabolism and up-regulate the expression of WRKYs in apple with bitter pit. Overexpression of MdWRKY75 significantly increased sucrose accumulation and the expression of MdSWEET1. These findings further strengthened knowledge of the basic molecular mechanisms in calcium deficiency apple flesh and contributed to improving the nutritional quality of apple fruit.
Identifiants
pubmed: 35176994
doi: 10.1186/s12870-022-03453-8
pii: 10.1186/s12870-022-03453-8
pmc: PMC8851858
doi:
Substances chimiques
Flavonoids
0
Phenols
0
Plant Proteins
0
Reactive Oxygen Species
0
Transcription Factors
0
Sucrose
57-50-1
Hydrogen Peroxide
BBX060AN9V
Ascorbic Acid
PQ6CK8PD0R
Calcium
SY7Q814VUP
Types de publication
Comparative Study
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
71Subventions
Organisme : Key Technologies Research and Development Program
ID : 2019YFD1000103
Organisme : National Natural Science Foundation of China
ID : 32170315
Organisme : National Natural Science Foundation of China
ID : 31901993
Organisme : National Natural Science Foundation of China
ID : 31970200
Organisme : Natural Science Foundations of Anhui Province
ID : 1908085MC72
Organisme : Fundamental Research Funds for the Central Universities
ID : JZ2021HGPA0063
Informations de copyright
© 2022. The Author(s).
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