Mediator complex subunit MED25 physically interacts with DST to regulate spikelet number in rice.
DST
MED25
OsCKX2
rice
spikelet number
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
04
11
2021
accepted:
24
02
2022
pubmed:
26
2
2022
medline:
20
4
2022
entrez:
25
2
2022
Statut:
ppublish
Résumé
Grain number is a flexible trait and contributes significantly to grain yield. In rice, the zinc finger transcription factor DROUGHT AND SALT TOLERANCE (DST) controls grain number by directly regulating cytokinin oxidase/dehydrogenase 2 (OsCKX2) expression. Although specific upstream regulators of the DST-OsCKX2 module have been identified, the mechanism employed by DST to regulate the expression of OsCKX2 remains unclear. Here, we demonstrate that DST-interacting protein 1 (DIP1), known as Mediator subunit OsMED25, acts as an interacting coactivator of DST. Phenotypic analyses revealed that OsMED25-RNAi and the osmed25 mutant plants exhibited enlarged panicles, with enhanced branching and spikelet number, similar to the dst mutant. Genetic analysis indicated that OsMED25 acts in the same pathway as the DST-OsCKX2 module to regulate spikelet number per panicle. Further biochemical analysis showed that OsMED25 physically interacts with DST at the promoter region of OsCKX2, and then recruits RNA polymerase II (Pol II) to activate OsCKX2 transcription. Thus, OsMED25 was involved in the communication between DST and Pol II general transcriptional machinery to regulate spikelet number. In general, our findings reveal a novel function of OsMED25 in DST-OsCKX2 modulated transcriptional regulation, thus enhancing our understanding of the regulatory mechanism underlying DST-OsCKX2-mediated spikelet number.
Substances chimiques
Mediator Complex
0
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
871-883Subventions
Organisme : National Key Research and Development Program of China
Organisme : Agricultural Seed Project of Shandong Province
Organisme : Tai-Shan Scholar Program from Shandong Province
Organisme : K. C. Wong Education Foundation
Informations de copyright
© 2022 Institute of Botany, Chinese Academy of Sciences.
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