Microscale thermophoresis and fluorescence polarization assays of calcineurin-peptide interactions.

Amino Acid Motifs Calcineurin / chemistry Calmodulin / metabolism Fluorescence Polarization Protein Binding
Affinity Calcineurin Fluorescence polarization Microscale thermophoresis Substrate

Journal

Analytical biochemistry
ISSN: 1096-0309
Titre abrégé: Anal Biochem
Pays: United States
ID NLM: 0370535

Informations de publication

Date de publication:
01 06 2022
Historique:
received: 13 11 2021
revised: 20 02 2022
accepted: 21 02 2022
pubmed: 27 2 2022
medline: 20 4 2022
entrez: 26 2 2022
Statut: ppublish

Résumé

Calcineurin is a Ca

Identifiants

DOI: 10.1016/j.ab.2022.114626 PMID: 35218735
pubmed: 35218735
pii: S0003-2697(22)00082-3
doi: 10.1016/j.ab.2022.114626
pii:
doi:

Substances chimiques

Calmodulin 0
Calcineurin EC 3.1.3.16

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

114626

Informations de copyright

Copyright © 2022 Elsevier Inc. All rights reserved.

Auteurs

Nan Zhang (N)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Yueyang Liu (Y)

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 111016, China.

Xiaoyu Shi (X)

College of Life Sciences, Langfang Normal University, Hebei, 065000, China.

Yuchen Zhang (Y)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Wenying Li (W)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Yumeng Yang (Y)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Limin Chen (L)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Yanxia Yin (Y)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Li Tong (L)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China.

Jingyu Yang (J)

Department of Pharmacology, Shenyang Pharmaceutical University, Shenyang, 111016, China. Electronic address: yangjingyu2006@gmail.com.

Jing Luo (J)

Department of Biochemistry and Molecular Biology, Gene Engineering and Biotechnology of Beijing Key Laboratory, College of Life Sciences, Beijing Normal University, Beijing, 100875, China. Electronic address: luojing@bnu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Structure secondaire des protéines › Motifs d'acides aminés › Microscale thermophoresis and fluorescence...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphoprotein Phosphatases › Calcineurine › Microscale thermophoresis and fluorescence...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Protéines intracellulaires sensibles au calcium › Calmoduline › Microscale thermophoresis and fluorescence...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Photométrie › Mesures de luminescence › Fluorimétrie › Polarisation de fluorescence › Microscale thermophoresis and fluorescence...
questionsmedicales.fr › Métabolisme › Liaison aux protéines › Microscale thermophoresis and fluorescence...