Investigation of salivary biomarkers as indicators of skeletal and dental maturity in children.
orthodontics
skeletal age
untargeted metabolomics
Journal
Orthodontics & craniofacial research
ISSN: 1601-6343
Titre abrégé: Orthod Craniofac Res
Pays: England
ID NLM: 101144387
Informations de publication
Date de publication:
Nov 2022
Nov 2022
Historique:
revised:
24
02
2022
received:
13
10
2021
accepted:
25
02
2022
pubmed:
18
3
2022
medline:
13
10
2022
entrez:
17
3
2022
Statut:
ppublish
Résumé
Estimation of patient's skeletal maturity in orthodontics is essential for the diagnosis and treatment planning. The aim of the study was to investigate the potential use of metabolic fingerprint of saliva for bone growth and tooth development estimation. Saliva samples from 54 young patients were analysed by an untargeted gas chromatography-mass spectrometry metabolomics-based method. The skeletal maturity was calculated with the cervical vertebrae maturation method, and the dental age was estimated with the Demirjian method. Multivariate analysis and univariate analysis were performed to investigate differences within skeletal, dental and chronological age groups. Metabolomic analysis identified 61 endogenous compounds. Mannose, glucose, glycerol, glyceric acid and pyroglutamic acid levels differentiated significantly with skeletal age (P = .02 to .043), while mannose, lactic acid, glycolic acid, proline, norleucine, 3-aminoisobutyric acid, threonine, cadaverine and hydrocinnamic acid levels differed within the dental age groups (P = .018 to .04); according to the chronological age, only the levels of mannose and 3-hydroxyphenylacetic acid showed variation (P = .029 and .048). The principal component analysis did not manage to highlight differences between the groups of the studied parameters. Differentiated levels of mannose, glucose, glycerol, glyceric acid and pyroglutamic acid related to skeletal maturation were identified. According to dental development, the levels of mannose, lactic acid, glycolic acid, proline, norleucine, 3-aminoisobutyric acid, threonine, cadaverine and hydrocinnamic acid differed within the groups, while regarding chronological age, only the levels of mannose and 3-hydroxyphenylacetic acid showed variations. Further studies are required to prove their relation to skeletal and dental development pathway by applying complementary analytical techniques to wider cover the metabolome.
Substances chimiques
Aminoisobutyric Acids
0
Biomarkers
0
Glyceric Acids
0
Glycolates
0
Phenylacetates
0
Phenylpropionates
0
glycolic acid
0WT12SX38S
Threonine
2ZD004190S
Lactic Acid
33X04XA5AT
3-phenylpropionic acid
5Q445IN5CU
glyceric acid
70KH64UX7G
Norleucine
832C8OV84S
Proline
9DLQ4CIU6V
Glucose
IY9XDZ35W2
3-hydroxybenzeneacetic acid
K59Z6Z8REF
Cadaverine
L90BEN6OLL
Glycerol
PDC6A3C0OX
Mannose
PHA4727WTP
Pyrrolidonecarboxylic Acid
SZB83O1W42
3-aminoisobutyric acid
T68ALE2O9F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
576-584Subventions
Organisme : Greece and the European Union (European Social Fund-ESF)
Informations de copyright
© 2022 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
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