Engineering Catharanthus roseus monoterpenoid indole alkaloid pathway in yeast.
Cancer chemotherapies
Catharanthus roseus
Heterologous expression
Metabolic engineering
Monoterpenoid indole alkaloids (MIAs)
Synthetic biology
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Apr 2022
Apr 2022
Historique:
received:
16
01
2022
accepted:
12
03
2022
revised:
07
03
2022
pubmed:
26
3
2022
medline:
12
4
2022
entrez:
25
3
2022
Statut:
ppublish
Résumé
Catharanthus roseus (Madagascar periwinkle), a medicinal plant possessing high pharmacological attributes, is widely recognized for the biosynthesis of anticancer monoterpenoid indole alkaloids (MIAs) - vinblastine and vincristine. The plant is known to biosynthesize more than 130 different bioactive MIAs, highly acclaimed in traditional and modern medicinal therapies. The MIA biosynthesis is strictly regulated at developmental and spatial-temporal stages and requires a well-defined cellular and sub-cellular compartmentation for completion of the entire MIAs biosynthesis. However, due to their cytotoxic nature, the production of vinblastine and vincristine occurs in low concentrations in planta and the absence of chemical synthesis alternatives projects a huge gap in demand and supply, leading to high market price. With research investigations spanning more than four decades, plant tissue culture and metabolic engineering (ME)-based studies were attempted to explore, understand, explain, improve and enhance the MIA biosynthesis using homologous and heterologous systems. Presently, metabolic engineering and synthetic biology are the two powerful tools that are contributing majorly in elucidating MIA biosynthesis. This review concentrates mainly on the efforts made through metabolic engineering of MIAs in heterologous microbial factories. KEY POINTS: • Yeast engineering provides alternative production source of phytomolecules • Yeast engineering also helps to discover missing plant pathway enzymes and genes.
Identifiants
pubmed: 35333954
doi: 10.1007/s00253-022-11883-5
pii: 10.1007/s00253-022-11883-5
doi:
Substances chimiques
Indole Alkaloids
0
Monoterpenes
0
Plant Proteins
0
Secologanin Tryptamine Alkaloids
0
Vincristine
5J49Q6B70F
Vinblastine
5V9KLZ54CY
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
2337-2347Subventions
Organisme : Science and Engineering Research Board
ID : SRG/2019/000130
Organisme : DST GSBTM
ID : GSBTM/JD (R&D)/610/2020-21/354
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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