Co-transcriptional splicing efficiency is a gene-specific feature that can be regulated by TGFβ.

Introns RNA Precursors / genetics RNA Splicing RNA, Messenger / genetics Transforming Growth Factor beta / genetics

Journal

Communications biology
ISSN: 2399-3642
Titre abrégé: Commun Biol
Pays: England
ID NLM: 101719179

Informations de publication

Date de publication:
28 03 2022
Historique:
received: 02 08 2021
accepted: 03 03 2022
entrez: 29 3 2022
pubmed: 30 3 2022
medline: 19 4 2022
Statut: epublish

Résumé

Differential splicing efficiency of specific introns is a mechanism that dramatically increases protein diversity, based on selection of alternative exons for the final mature mRNA. However, it is unclear whether splicing efficiency of introns within the same gene is coordinated and eventually regulated as a mechanism to control mature mRNA levels. Based on nascent chromatin-associated RNA-sequencing data, we now find that co-transcriptional splicing (CTS) efficiency tends to be similar between the different introns of a gene. We establish that two well-differentiated strategies for CTS efficiency exist, at the extremes of a gradient: short genes that produce high levels of pre-mRNA undergo inefficient splicing, while long genes with relatively low levels of pre-mRNA have an efficient splicing. Notably, we observe that genes with efficient CTS display a higher level of mature mRNA relative to their pre-mRNA levels. Further, we show that the TGFβ signal transduction pathway regulates the general CTS efficiency, causing changes in mature mRNA levels. Taken together, our data indicate that CTS efficiency is a gene-specific characteristic that can be regulated to control gene expression.

Identifiants

DOI: 10.1038/s42003-022-03224-z PMID: 35347226 PMC: PMC8960766
pubmed: 35347226
doi: 10.1038/s42003-022-03224-z
pii: 10.1038/s42003-022-03224-z
pmc: PMC8960766
doi:

Substances chimiques

RNA Precursors 0
RNA, Messenger 0
Transforming Growth Factor beta 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

277

Subventions

Organisme : Ministry of Economy and Competitiveness | Agencia Estatal de Investigación (Spanish Agencia Estatal de Investigación)
ID : PID2020-118516GB-I00
Organisme : Ministry of Economy and Competitiveness | Agencia Estatal de Investigación (Spanish Agencia Estatal de Investigación)
ID : BFU2017-85420-R
Organisme : Consejería de Economía, Innovación, Ciencia y Empleo, Junta de Andalucía (Ministry of Economy, Innovation, Science and Employment, Government of Andalucia)
ID : P18-FR-1962

Informations de copyright

© 2022. The Author(s).

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Auteurs

Elena Sánchez-Escabias (E)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla-Universidad Pablo de Olavide (CSIC-USE-UPO), Avenida Americo Vespucio, 41092, Seville, Spain.

José A Guerrero-Martínez (JA)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla-Universidad Pablo de Olavide (CSIC-USE-UPO), Avenida Americo Vespucio, 41092, Seville, Spain. jose.guerrero@cabimer.es.

José C Reyes (JC)

Centro Andaluz de Biología Molecular y Medicina Regenerativa-CABIMER, Consejo Superior de Investigaciones Científicas-Universidad de Sevilla-Universidad Pablo de Olavide (CSIC-USE-UPO), Avenida Americo Vespucio, 41092, Seville, Spain. jose.reyes@cabimer.es.
ORCID: 0000-0002-8042-5142

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Classifications MeSH

questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Introns › Co-transcriptional splicing efficiency is a...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › Précurseurs des ARN › Co-transcriptional splicing efficiency is a...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Maturation post-transcriptionnelle des ARN › Épissage des ARN › Co-transcriptional splicing efficiency is a...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN messager › Co-transcriptional splicing efficiency is a...
questionsmedicales.fr › Facteurs biologiques › Protéines et peptides de signalisation intercellulaire › Protéines de la superfamille du TGF bêta › Facteur de croissance transformant bêta › Co-transcriptional splicing efficiency is a...