The complete sequence of a human genome.


Journal

Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511

Informations de publication

Date de publication:
04 2022
Historique:
entrez: 31 3 2022
pubmed: 1 4 2022
medline: 6 4 2022
Statut: ppublish

Résumé

Since its initial release in 2000, the human reference genome has covered only the euchromatic fraction of the genome, leaving important heterochromatic regions unfinished. Addressing the remaining 8% of the genome, the Telomere-to-Telomere (T2T) Consortium presents a complete 3.055 billion-base pair sequence of a human genome, T2T-CHM13, that includes gapless assemblies for all chromosomes except Y, corrects errors in the prior references, and introduces nearly 200 million base pairs of sequence containing 1956 gene predictions, 99 of which are predicted to be protein coding. The completed regions include all centromeric satellite arrays, recent segmental duplications, and the short arms of all five acrocentric chromosomes, unlocking these complex regions of the genome to variational and functional studies.

Identifiants

pubmed: 35357919
doi: 10.1126/science.abj6987
pmc: PMC9186530
mid: NIHMS1775562
doi:

Types de publication

Journal Article Research Support, N.I.H., Intramural Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

44-53

Subventions

Organisme : NHGRI NIH HHS
ID : U01 HG010961
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM130151
Pays : United States
Organisme : NIMH NIH HHS
ID : DP2 MH119424
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA240199
Pays : United States
Organisme : Intramural NIH HHS
ID : Z99 HG999999
Pays : United States
Organisme : NHGRI NIH HHS
ID : U24 HG009081
Pays : United States
Organisme : NCI NIH HHS
ID : R21 CA238758
Pays : United States
Organisme : NHGRI NIH HHS
ID : U01 HG010971
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM123312
Pays : United States
Organisme : NHGRI NIH HHS
ID : UM1 HG008898
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG002385
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA HG200398
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM124041
Pays : United States
Organisme : NHGRI NIH HHS
ID : U24 HG010263
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG006677
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM133747
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007445
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG010040
Pays : United States
Organisme : NHGRI NIH HHS
ID : U41 HG010972
Pays : United States
Organisme : NHGRI NIH HHS
ID : F31 HG011205
Pays : United States
Organisme : NIGMS NIH HHS
ID : F32 GM134558
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG010169
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM129263
Pays : United States
Organisme : NHGRI NIH HHS
ID : U41 HG007234
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA253481
Pays : United States
Organisme : NHGRI NIH HHS
ID : U24 HG007234
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG011274
Pays : United States
Organisme : NHGRI NIH HHS
ID : U24 HG006620
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG002939
Pays : United States
Organisme : NIA NIH HHS
ID : R01 AG054712
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG009190
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG010485
Pays : United States
Organisme : NIDDK NIH HHS
ID : R24 DK106766
Pays : United States

Commentaires et corrections

Type : CommentIn
Type : CommentIn
Type : CommentIn
Type : CommentIn

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Auteurs

Sergey Nurk (S)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Sergey Koren (S)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Arang Rhie (A)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Mikko Rautiainen (M)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Andrey V Bzikadze (AV)

Graduate Program in Bioinformatics and Systems Biology, University of California, San Diego, La Jolla, CA, USA.

Alla Mikheenko (A)

Center for Algorithmic Biotechnology, Institute of Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.

Mitchell R Vollger (MR)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Nicolas Altemose (N)

Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.

Lev Uralsky (L)

Sirius University of Science and Technology, Sochi, Russia.
Vavilov Institute of General Genetics, Moscow, Russia.

Ariel Gershman (A)

Department of Molecular Biology and Genetics, Johns Hopkins University, Baltimore, MD, USA.

Sergey Aganezov (S)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.

Savannah J Hoyt (SJ)

Institute for Systems Genomics and Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.

Mark Diekhans (M)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.

Glennis A Logsdon (GA)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Michael Alonge (M)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.

Stylianos E Antonarakis (SE)

University of Geneva Medical School, Geneva, Switzerland.

Matthew Borchers (M)

Stowers Institute for Medical Research, Kansas City, MO, USA.

Gerard G Bouffard (GG)

NIH Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Shelise Y Brooks (SY)

NIH Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Gina V Caldas (GV)

Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, CA, USA.

Nae-Chyun Chen (NC)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.

Haoyu Cheng (H)

Department of Data Sciences, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.

Chen-Shan Chin (CS)

DNAnexus, Mountain View, CA, USA.

William Chow (W)

Wellcome Sanger Institute, Cambridge, UK.

Leonardo G de Lima (LG)

Stowers Institute for Medical Research, Kansas City, MO, USA.

Philip C Dishuck (PC)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Richard Durbin (R)

Wellcome Sanger Institute, Cambridge, UK.
Department of Genetics, University of Cambridge, Cambridge, UK.

Tatiana Dvorkina (T)

Center for Algorithmic Biotechnology, Institute of Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.

Ian T Fiddes (IT)

Inscripta, Boulder, CO, USA.

Giulio Formenti (G)

Laboratory of Neurogenetics of Language and The Vertebrate Genome Lab, The Rockefeller University, New York, NY, USA.
Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA.

Robert S Fulton (RS)

Department of Genetics, Washington University School of Medicine, St. Louis, MO, USA.

Erik Garrison (E)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
University of Tennessee Health Science Center, Memphis, TN, USA.

Patrick G S Grady (PGS)

Institute for Systems Genomics and Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.

Tina A Graves-Lindsay (TA)

McDonnell Genome Institute, Washington University in St. Louis, St. Louis, MO, USA.

Ira M Hall (IM)

Department of Genetics, Yale University School of Medicine, New Haven, CT, USA.

Nancy F Hansen (NF)

Comparative Genomics Analysis Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Gabrielle A Hartley (GA)

Institute for Systems Genomics and Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.

Marina Haukness (M)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.

Kerstin Howe (K)

Wellcome Sanger Institute, Cambridge, UK.

Michael W Hunkapiller (MW)

Pacific Biosciences, Menlo Park, CA, USA.

Chirag Jain (C)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
Department of Computational and Data Sciences, Indian Institute of Science, Bangalore KA, India.

Miten Jain (M)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.

Erich D Jarvis (ED)

Laboratory of Neurogenetics of Language and The Vertebrate Genome Lab, The Rockefeller University, New York, NY, USA.
Howard Hughes Medical Institute, The Rockefeller University, New York, NY, USA.

Peter Kerpedjiev (P)

Reservoir Genomics LLC, Oakland, CA, USA.

Melanie Kirsche (M)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.

Mikhail Kolmogorov (M)

Department of Computer Science and Engineering, University of California, San Diego, La Jolla, CA, USA.

Jonas Korlach (J)

Pacific Biosciences, Menlo Park, CA, USA.

Milinn Kremitzki (M)

McDonnell Genome Institute, Washington University in St. Louis, St. Louis, MO, USA.

Heng Li (H)

Department of Data Sciences, Dana-Farber Cancer Institute, Boston, MA, USA.
Department of Biomedical Informatics, Harvard Medical School, Boston, MA, USA.

Valerie V Maduro (VV)

Undiagnosed Diseases Program, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Tobias Marschall (T)

Heinrich Heine University Düsseldorf, Medical Faculty, Institute for Medical Biometry and Bioinformatics, Düsseldorf, Germany.

Ann M McCartney (AM)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Jennifer McDaniel (J)

Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.

Danny E Miller (DE)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.
Department of Pediatrics, Division of Genetic Medicine, University of Washington and Seattle Children's Hospital, Seattle, WA, USA.

James C Mullikin (JC)

NIH Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.
Comparative Genomics Analysis Unit, Cancer Genetics and Comparative Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Eugene W Myers (EW)

Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany.

Nathan D Olson (ND)

Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.

Benedict Paten (B)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.

Paul Peluso (P)

Pacific Biosciences, Menlo Park, CA, USA.

Pavel A Pevzner (PA)

Department of Computer Science and Engineering, University of California, San Diego, La Jolla, CA, USA.

David Porubsky (D)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.

Tamara Potapova (T)

Stowers Institute for Medical Research, Kansas City, MO, USA.

Evgeny I Rogaev (EI)

Sirius University of Science and Technology, Sochi, Russia.
Vavilov Institute of General Genetics, Moscow, Russia.
Department of Psychiatry, University of Massachusetts Medical School, Worcester, MA, USA.
Faculty of Biology, Lomonosov Moscow State University, Moscow, Russia.

Jeffrey A Rosenfeld (JA)

Cancer Institute of New Jersey, New Brunswick, NJ, USA.

Steven L Salzberg (SL)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Valerie A Schneider (VA)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.

Fritz J Sedlazeck (FJ)

Human Genome Sequencing Center, Baylor College of Medicine, Houston, TX, USA.

Kishwar Shafin (K)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.

Colin J Shew (CJ)

Genome Center, MIND Institute, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.

Alaina Shumate (A)

Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Ying Sims (Y)

Wellcome Sanger Institute, Cambridge, UK.

Arian F A Smit (AFA)

Institute for Systems Biology, Seattle, WA, USA.

Daniela C Soto (DC)

Genome Center, MIND Institute, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.

Ivan Sović (I)

Pacific Biosciences, Menlo Park, CA, USA.
Digital BioLogic d.o.o., Ivanić-Grad, Croatia.

Jessica M Storer (JM)

Institute for Systems Biology, Seattle, WA, USA.

Aaron Streets (A)

Department of Bioengineering, University of California, Berkeley, Berkeley, CA, USA.
Chan Zuckerberg Biohub, San Francisco, CA, USA.

Beth A Sullivan (BA)

Department of Molecular Genetics and Microbiology, Duke University School of Medicine, Durham, NC, USA.

Françoise Thibaud-Nissen (F)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.

James Torrance (J)

Wellcome Sanger Institute, Cambridge, UK.

Justin Wagner (J)

Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.

Brian P Walenz (BP)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Aaron Wenger (A)

Pacific Biosciences, Menlo Park, CA, USA.

Jonathan M D Wood (JMD)

Wellcome Sanger Institute, Cambridge, UK.

Chunlin Xiao (C)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD, USA.

Stephanie M Yan (SM)

Department of Biology, Johns Hopkins University, Baltimore, MD, USA.

Alice C Young (AC)

NIH Intramural Sequencing Center, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

Samantha Zarate (S)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.

Urvashi Surti (U)

Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA.

Rajiv C McCoy (RC)

Department of Biology, Johns Hopkins University, Baltimore, MD, USA.

Megan Y Dennis (MY)

Genome Center, MIND Institute, Department of Biochemistry and Molecular Medicine, University of California, Davis, CA, USA.

Ivan A Alexandrov (IA)

Center for Algorithmic Biotechnology, Institute of Translational Biomedicine, Saint Petersburg State University, Saint Petersburg, Russia.
Vavilov Institute of General Genetics, Moscow, Russia.
Research Center of Biotechnology of the Russian Academy of Sciences, Moscow, Russia.

Jennifer L Gerton (JL)

Stowers Institute for Medical Research, Kansas City, MO, USA.
Department of Biochemistry and Molecular Biology, University of Kansas Medical School, Kansas City, MO, USA.

Rachel J O'Neill (RJ)

Institute for Systems Genomics and Department of Molecular and Cell Biology, University of Connecticut, Storrs, CT, USA.

Winston Timp (W)

Department of Molecular Biology and Genetics, Johns Hopkins University, Baltimore, MD, USA.
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD, USA.

Justin M Zook (JM)

Biosystems and Biomaterials Division, National Institute of Standards and Technology, Gaithersburg, MD, USA.

Michael C Schatz (MC)

Department of Computer Science, Johns Hopkins University, Baltimore, MD, USA.
Department of Biology, Johns Hopkins University, Baltimore, MD, USA.

Evan E Eichler (EE)

Department of Genome Sciences, University of Washington School of Medicine, Seattle, WA, USA.
Howard Hughes Medical Institute, University of Washington, Seattle, WA, USA.

Karen H Miga (KH)

UC Santa Cruz Genomics Institute, University of California, Santa Cruz, Santa Cruz, CA, USA.
Department of Biomolecular Engineering, University of California, Santa Cruz, Santa Cruz, CA, USA.

Adam M Phillippy (AM)

Genome Informatics Section, Computational and Statistical Genomics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, MD, USA.

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Jun Hwan Cho, Seung Yong Shin, Hoseob Kim et al.
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Humans Male Smoking Cessation Cardiovascular Diseases Female
Humans United States Aged Cross-Sectional Studies Medicare Part C

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