Transition metal dichalcogenide-based Janus micromotors for on-the-fly Salmonella detection.
Affinity peptides
Endotoxins
Fluorescence
Janus
Micromotors
Journal
Mikrochimica acta
ISSN: 1436-5073
Titre abrégé: Mikrochim Acta
Pays: Austria
ID NLM: 7808782
Informations de publication
Date de publication:
15 04 2022
15 04 2022
Historique:
received:
18
01
2022
accepted:
01
04
2022
entrez:
15
4
2022
pubmed:
16
4
2022
medline:
19
4
2022
Statut:
epublish
Résumé
Janus micromotors encapsulating transition metal dichalcogenides (TMDs) and modified with a rhodamine (RhO)-labeled affinity peptide (RhO-NFMESLPRLGMH) are used here for Salmonella enterica endotoxin detection. The OFF-ON strategy relies on the specific binding of the peptide with the TMDs to induce fluorescence quenching (OFF state); which is next recovered due to selectively binding to the endotoxin (ON state). The increase in the fluorescence of the micromotors can be quantified as a function of the concentration of endotoxin in the sample. The developed strategy was applied to the determination of Salmonella enterica serovar Typhimurium endotoxin with high sensitivity (limits of detection (LODs) of 2.0 µg/mL using MoS
Identifiants
pubmed: 35426053
doi: 10.1007/s00604-022-05298-2
pii: 10.1007/s00604-022-05298-2
pmc: PMC9010330
doi:
Substances chimiques
Endotoxins
0
Peptides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
194Subventions
Organisme : Ministerio de Ciencia e Innovación
ID : RYC-2015-17558
Organisme : Ministerio de Ciencia e Innovación
ID : PID2020-118154GB-I00
Organisme : Ministerio de Ciencia e Innovación
ID : PID2020-118154GB-I00
Organisme : Comunidad de Madrid
ID : TRANSNANOAVANSENS
Organisme : Comunidad de Madrid
ID : S2018/NMT-4349
Organisme : Comunidad de Madrid
ID : CM/JIN/2019-007
Informations de copyright
© 2022. The Author(s).
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