Quantifying Phytohormones in Vivo with FRET Biosensors and the FRETENATOR Analysis Toolset.

Biosensing Techniques / methods Fluorescence Resonance Energy Transfer / methods Gibberellins Hormones Plant Growth Regulators
ABA Abscisic acid Biosensors FRET GA Gibberellin Image analysis Microscopy

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 25 4 2022
pubmed: 26 4 2022
medline: 28 4 2022
Statut: ppublish

Résumé

The ABACUS1-2 μ (ABscisic Acid Concentration and Uptake Sensor 1-2 μ) and GPS1 (Gibberellin Perception Sensor 1) are direct Förster resonance energy transfer (FRET) biosensors that can be used to measure hormone levels in planta. We provide detailed protocols to image FRET biosensors under exogenously applied hormones in roots, either as a single time point or for treatment time courses before and after hormone application. A new, free, open-source analysis toolset for Fiji is introduced and used to get full 3D segmentation of images of nuclear localized FRET biosensors and calculate emission ratios on a per nucleus basis allowing in-depth analysis of biosensor data.

Identifiants

DOI: 10.1007/978-1-0716-2297-1_17 PMID: 35467212
pubmed: 35467212
doi: 10.1007/978-1-0716-2297-1_17
doi:

Substances chimiques

Gibberellins 0
Hormones 0
Plant Growth Regulators 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

239-253

Subventions

Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P018572/1
Pays : United Kingdom

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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doi: 10.1126/science.1118642 pubmed: 16400150
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doi: 10.1105/tpc.112.107227 pubmed: 23341337 pmcid: 3584545
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doi: 10.1146/annurev-arplant-042817-040104 pubmed: 29719164
Herud-Sikimić O, Stiel AC, Kolb M, Shanmugaratnam S, Berendzen KW, Feldhaus C, Höcker B, Jürgens G (2021) A biosensor for the direct visualization of auxin. Nature 592:768–772. https://doi.org/10.1038/s41586-021-03425-2
doi: 10.1038/s41586-021-03425-2 pubmed: 33828298 pmcid: 8081663
Jones AM, Danielson JÅH, ManojKumar SN, Lanquar V, Grossmann G, Frommer WB (2014) Abscisic acid dynamics in roots detected with genetically encoded FRET sensors. elife 3:e01741
doi: 10.7554/eLife.01741
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Schindelin J, Arganda-Carreras I, Frise E, Kaynig V, Longair M, Pietzsch T, Preibisch S, Rueden C, Saalfeld S, Schmid B (2012) Fiji: an open-source platform for biological-image analysis. Nat Methods 9:676
doi: 10.1038/nmeth.2019
Rizza A, Walia A, Tang B, Jones AM (2019) Visualizing cellular gibberellin levels using the nlsGPS1 Förster resonance energy transfer (FRET) biosensor. J Vis Exp 2019:58739 . https://doi.org/ https://doi.org/10.3791/58739
Haase R, Royer LA, Steinbach P, Schmidt D, Dibrov A, Schmidt U, Weigert M, Maghelli N, Tomancak P, Jug F, Myers EW (2020) CLIJ: GPU-accelerated image processing for everyone. Nat Methods 17:5–6
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Auteurs

James H Rowe (JH)

Sainsbury Laboratory, University of Cambridge, Cambridge, UK.

Annalisa Rizza (A)

Sainsbury Laboratory, University of Cambridge, Cambridge, UK.

Alexander M Jones (AM)

Sainsbury Laboratory, University of Cambridge, Cambridge, UK. alexander.jones@slcu.cam.ac.uk.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de sonde moléculaire › Techniques de biocapteur › Quantifying Phytohormones in Vivo with FRET...
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Transfert d'énergie par résonance de fluorescence › Quantifying Phytohormones in Vivo with FRET...
questionsmedicales.fr › Composés hétérocycliques › Alcaloïdes › Alcaloïdes diterpéniques › Diterpènes de type kaurane › Gibbérellines › Quantifying Phytohormones in Vivo with FRET...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Effets physiologiques des médicaments › Hormones, substituts d'hormones et antagonistes d'hormones › Hormones › Quantifying Phytohormones in Vivo with FRET...
questionsmedicales.fr › Actions chimiques et utilisations › Actions pharmacologiques › Effets physiologiques des médicaments › Substances de croissance › Facteur de croissance végétal › Quantifying Phytohormones in Vivo with FRET...