Genome-wide identification and expression analysis of magnesium transporter gene family in grape (Vitis vinifera).

Arabidopsis / genetics Gene Expression Regulation, Plant Magnesium / metabolism Membrane Transport Proteins / metabolism Phylogeny Plant Proteins / metabolism Vitis / metabolism
Gene expression Grapes MGTs Phylogenetic analysis

Journal

BMC plant biology
ISSN: 1471-2229
Titre abrégé: BMC Plant Biol
Pays: England
ID NLM: 100967807

Informations de publication

Date de publication:
28 Apr 2022
Historique:
received: 16 01 2022
accepted: 14 04 2022
entrez: 28 4 2022
pubmed: 29 4 2022
medline: 30 4 2022
Statut: epublish

Résumé

Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of magnesium ion transporters (MGT) is helpful to understand the dynamic balance mechanism of intracellular magnesium ions and their inter- or intra-cellular activities. In this study, we identified the members of MGT protein family in grape and performed the phylogenetic and expression analysis. We have identified nine VvMGT genes in grape genome, which are distributed on eight different chromosomes. Phylogenetic analysis showed that MGT family members of grapes were divided into five subfamilies and had obvious homology with Arabidopsis, maize, and pear. Based on transcriptome data from the web databases, we analyzed the expression patterns of VvMGTs at different development stages and in response to abiotic stresses including waterlogging, drought, salinity, and copper. Using qRT-PCR method, we tested the expression of grape VvMGTs under magnesium and aluminum treatments and found significant changes in VvMGTs expression. In addition, four of the MGT proteins in grape were located in the nucleus. Overall, in this study we investigated the structural characteristics, evolution pattern, and expression analysis of VvMGTs in depth, which laid the foundation for further revealing the function of VvMGT genes in grape.

Sections du résumé

BACKGROUND BACKGROUND
Magnesium ion is one of the essential mineral elements for plant growth and development, which participates in a variety of physiological and biochemical processes. Since there is no report on the research of magnesium ion transporter in grape, the study of the structure and function of magnesium ion transporters (MGT) is helpful to understand the dynamic balance mechanism of intracellular magnesium ions and their inter- or intra-cellular activities.
RESULT RESULTS
In this study, we identified the members of MGT protein family in grape and performed the phylogenetic and expression analysis. We have identified nine VvMGT genes in grape genome, which are distributed on eight different chromosomes. Phylogenetic analysis showed that MGT family members of grapes were divided into five subfamilies and had obvious homology with Arabidopsis, maize, and pear. Based on transcriptome data from the web databases, we analyzed the expression patterns of VvMGTs at different development stages and in response to abiotic stresses including waterlogging, drought, salinity, and copper. Using qRT-PCR method, we tested the expression of grape VvMGTs under magnesium and aluminum treatments and found significant changes in VvMGTs expression. In addition, four of the MGT proteins in grape were located in the nucleus.
CONCLUSION CONCLUSIONS
Overall, in this study we investigated the structural characteristics, evolution pattern, and expression analysis of VvMGTs in depth, which laid the foundation for further revealing the function of VvMGT genes in grape.

Identifiants

DOI: 10.1186/s12870-022-03599-5 PMID: 35477360 PMC: PMC9047265
pubmed: 35477360
doi: 10.1186/s12870-022-03599-5
pii: 10.1186/s12870-022-03599-5
pmc: PMC9047265
doi:

Substances chimiques

Membrane Transport Proteins 0
Plant Proteins 0
Magnesium I38ZP9992A

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

217

Informations de copyright

© 2022. The Author(s).

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Auteurs

Mengqing Ge (M)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Rong Zhong (R)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Ehsan Sadeghnezhad (E)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Abdul Hakeem (A)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Xin Xiao (X)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Peipei Wang (P)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China.

Jinggui Fang (J)

College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China. 2017104012@njau.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Brassicaceae › Arabidopsis › Genome-wide identification and expression...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › Genome-wide identification and expression...
questionsmedicales.fr › Produits chimiques inorganiques › Métaux › Métaux légers › Magnésium › Genome-wide identification and expression...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines membranaires › Protéines de transport membranaire › Genome-wide identification and expression...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Genome-wide identification and expression...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › Genome-wide identification and expression...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Vitaceae › Vitis › Genome-wide identification and expression...