Donor manipulation for constructing a pH sensing thermally activated delayed fluorescent probe to detect alkaliphiles.

Biotechnology Fluorescence Fluorescent Dyes / chemistry Hydrogen-Ion Concentration
Alkaliphiles Sensor Thermally activated delayed fluorescence Time-resolved fluorescence imaging pH

Journal

Talanta
ISSN: 1873-3573
Titre abrégé: Talanta
Pays: Netherlands
ID NLM: 2984816R

Informations de publication

Date de publication:
15 Aug 2022
Historique:
received: 18 02 2022
revised: 13 04 2022
accepted: 17 04 2022
pubmed: 1 5 2022
medline: 10 6 2022
entrez: 30 4 2022
Statut: ppublish

Résumé

pH homeostasis is essential for alkaliphiles, given their widespread use in biotechnological applications. However, quantitative monitoring of alkaline pH in alkaliphiles remains challenging. Here, we synthesized for the first time, a thermally activated delayed fluorescent (TADF) pH probe: NI-D-OH. Our probe exhibits a good linear relationship between fluorescence intensity and pH in the neutral to alkaline range (pH 7.0-8.6), as well as long-lived TADF emission. We further show that NI-D-OH can be used to monitor intracellular pH in living organisms, and evaluate the effect of Na

Identifiants

DOI: 10.1016/j.talanta.2022.123493 PMID: 35489098
pubmed: 35489098
pii: S0039-9140(22)00289-2
doi: 10.1016/j.talanta.2022.123493
pii:
doi:

Substances chimiques

Fluorescent Dyes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

123493

Informations de copyright

Copyright © 2022 Elsevier B.V. All rights reserved.

Auteurs

Qian Liu (Q)

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.

Miao Yang (M)

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China; School of Pharmaceutical Engineering and Key Laboratory of Structure-Based Drug Design & Discovery (Ministry of Education), Shenyang Pharmaceutical University, Shenyang, 110016, China.

Xiangchuan Meng (X)

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.

Xiaowan Han (X)

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China.

Marc Nazare (M)

Medicinal Chemistry, Leibniz-Forschungsinstitut für Molekulare Pharmakologie Berlin, 13125, Berlin, Germany.

Youjun Xu (Y)

School of Pharmaceutical Engineering and Key Laboratory of Structure-Based Drug Design & Discovery (Ministry of Education), Shenyang Pharmaceutical University, Shenyang, 110016, China. Electronic address: xuyoujun@syphu.edu.cn.

Hai-Yu Hu (HY)

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China. Electronic address: haiyu.hu@imm.ac.cn.

Qingyang Zhang (Q)

State Key Laboratory of Bioactive Substances and Function of Natural Medicine, Beijing Key Laboratory of Active Substances Discovery and Drugability Evaluation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing, 100050, China. Electronic address: zhangqingyang@imm.ac.cn.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Technologie › Biotechnologie › Donor manipulation for constructing a pH...
questionsmedicales.fr › Phénomènes physiques › Phénomènes optiques › Fluorescence › Donor manipulation for constructing a pH...
questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Donor manipulation for constructing a pH...
questionsmedicales.fr › Phénomènes chimiques › Concentration en ions d'hydrogène › Donor manipulation for constructing a pH...