Separation and determination of basic orange II, acid orange II and auramine O in soybean products based on ionic liquid reverse micelle microextraction and ultra-high-performance liquid chromatography.

A new analytical method was developed for the separation and determination of basic orange II, acid orange II and auramine O in soybean products. The technique was focused on ionic liquid reverse micelle microextraction (IL-RMME) followed by analysis and determination by ultra-high performance liquid chromatography (UHPLC) with photodiode array detector of three chemical dyes. In this method, IL-RMME solution consisting of ionic liquid [Omim]BF6 and surfactant GenapolX-080 was used as extractant. Important parameters affecting IL-RMME efficiency, such as extraction solvent type and volume, sample solution pH, salt effect, centrifugation speed and time were investigated. Under the optimal condition, the linearity of the method was in the range of 0.1-10 ng mL

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Declaration of competing interest We declare that we have no financial and personal relationships with other people or organizations that can inappropriately influence our work, there is no professional or other personal interest of any nature or kind in any product, service and/or company that could be construed as influencing the position presented in, or the review of, the manuscript entitled.