Glycerol-3-Phosphate Dehydrogenase: The K120 and K204 Side Chains Define an Oxyanion Hole at the Enzyme Active Site.
Journal
Biochemistry
ISSN: 1520-4995
Titre abrégé: Biochemistry
Pays: United States
ID NLM: 0370623
Informations de publication
Date de publication:
17 05 2022
17 05 2022
Historique:
pubmed:
4
5
2022
medline:
20
5
2022
entrez:
3
5
2022
Statut:
ppublish
Résumé
The cationic K120 and K204 side chains lie close to the C-2 carbonyl group of substrate dihydroxyacetone phosphate (DHAP) at the active site of glycerol-3-phosphate dehydrogenase (GPDH), and the K120 side chain is also positioned to form a hydrogen bond to the C-1 hydroxyl of DHAP. The kinetic parameters for unactivated and phosphite dianion-activated GPDH-catalyzed reduction of glycolaldehyde and acetaldehyde (AcA) show that the transition state for the former reaction is stabilized by
Identifiants
pubmed: 35502876
doi: 10.1021/acs.biochem.2c00053
pmc: PMC9119304
doi:
Substances chimiques
Dihydroxyacetone Phosphate
57-04-5
Glycerolphosphate Dehydrogenase
EC 1.1.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
856-867Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM116921
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM134881
Pays : United States
Références
J Am Chem Soc. 2015 Jan 28;137(3):1372-82
pubmed: 25555107
Proc Natl Acad Sci U S A. 2010 May 25;107(21):9572-7
pubmed: 20457944
J Am Chem Soc. 2010 Sep 29;132(38):13525-32
pubmed: 20822141
Biochemistry. 2007 May 15;46(19):5841-54
pubmed: 17444661
Biochemistry. 2020 Dec 29;59(51):4856-4863
pubmed: 33305938
Biochemistry. 2019 Feb 26;58(8):1061-1073
pubmed: 30640445
Annu Rev Biochem. 2010;79:471-505
pubmed: 20235827
J Am Chem Soc. 2015 Apr 29;137(16):5312-5
pubmed: 25884759
J Am Chem Soc. 2004 Apr 28;126(16):5044-5
pubmed: 15099068
J Mol Biol. 2003 May 30;329(2):335-49
pubmed: 12758080
Biochem J. 1971 Apr;122(3):285-97
pubmed: 4330197
J Am Chem Soc. 2001 Nov 14;123(45):11262-72
pubmed: 11697969
Bioorg Chem. 2004 Oct;32(5):341-53
pubmed: 15381400
Acc Chem Res. 2021 May 18;54(10):2532-2542
pubmed: 33939414
Adv Exp Med Biol. 1993;328:391-400
pubmed: 8493917
J Am Chem Soc. 2019 Feb 27;141(8):3320-3331
pubmed: 30703322
Biochemistry. 2020 Apr 28;59(16):1582-1591
pubmed: 32250105
J Am Chem Soc. 2016 Nov 23;138(46):15251-15259
pubmed: 27792325
J Am Chem Soc. 2016 Nov 9;138(44):14526-14529
pubmed: 27769116
Curr Opin Chem Biol. 2010 Apr;14(2):200-7
pubmed: 20080434
J Am Chem Soc. 2002 Sep 4;124(35):10572-7
pubmed: 12197759
J Am Chem Soc. 2001 Oct 10;123(40):9912-3
pubmed: 11583562
Biochemistry. 2016 Mar 15;55(10):1429-32
pubmed: 26926520
Biochemistry. 2008 Apr 22;47(16):4575-82
pubmed: 18376850
Nucleic Acids Res. 2003 Jul 1;31(13):3784-8
pubmed: 12824418
J Am Chem Soc. 2021 Feb 24;143(7):2694-2698
pubmed: 33560827
Biochemistry. 2018 Jul 24;57(29):4338-4348
pubmed: 29927590
Biochemistry. 2000 Feb 8;39(5):903-9
pubmed: 10653633
Biochemistry. 2013 Mar 26;52(12):2009-11
pubmed: 23452047
Curr Opin Chem Biol. 2014 Aug;21:1-10
pubmed: 24699188
Biochemistry. 1995 Jan 17;34(2):464-71
pubmed: 7819238
Biochemistry. 2009 Jun 23;48(24):5769-78
pubmed: 19425580
Nat Chem Biol. 2009 Aug;5(8):543-50
pubmed: 19620995
Biochemistry. 2018 Jun 12;57(23):3227-3236
pubmed: 29337541
J Am Chem Soc. 2011 Dec 21;133(50):20052-5
pubmed: 22053826
J Biol Chem. 1998 Oct 16;273(42):27035-8
pubmed: 9765214
Biochemistry. 1991 Sep 17;30(37):8924-8
pubmed: 1892809