Cellulose accumulation mediated by PbrCSLD5, a cellulose synthase-like protein, results in cessation of pollen tube growth in Pyrus bretschneideri.
Journal
Physiologia plantarum
ISSN: 1399-3054
Titre abrégé: Physiol Plant
Pays: Denmark
ID NLM: 1256322
Informations de publication
Date de publication:
May 2022
May 2022
Historique:
revised:
21
04
2022
received:
14
10
2021
accepted:
05
05
2022
pubmed:
9
5
2022
medline:
30
6
2022
entrez:
8
5
2022
Statut:
ppublish
Résumé
Cellulose, a key component of the cell wall, plays an important role in maintaining the growth of pollen tubes. However, the molecular mechanism of cellulose participating in the cessation of pear pollen tube growth remains unclear. Here, we reported that at 15 h post-cultured (HPC), the slow-growth pear pollen tubes showed thickened cell walls and cellulose accumulation in the inner wall. Transcriptome data and quantitative real-time PCR analysis showed that PbrCSLD5, a cellulose synthesis-like gene, was highly expressed in the 15 HPC pear pollen tubes. Knockdown of PbrCSLD5 caused a decrease in cellulose content in pear pollen tubes. Moreover, PbrCSLD5 overexpression in Arabidopsis resulted in the accumulation of cellulose and disruption of normal pollen tube growth. Transcription factor PbrMADS52 was found to bind to the promoter of PbrCSLD5 and enhanced its expression. Our results suggested that the PbrMADS52-PbrCSLD5 signaling pathway led to increased cellulose content in the pear pollen tube cell wall, thereby inhibiting pollen tube growth. These results provided new insights into the regulation of pollen tube growth.
Substances chimiques
Plant Proteins
0
Cellulose
9004-34-6
Glucosyltransferases
EC 2.4.1.-
cellulose synthase
EC 2.4.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13700Subventions
Organisme : National Natural Science Foundation of China
ID : 32102358
Organisme : National Natural Science Foundation of China
ID : 32172543
Organisme : Natural Science Foundation of Jiangsu Province
ID : BK20210394
Organisme : Priority Academic Program Development of Jiangsu Higher Education Institutions
Informations de copyright
© 2022 Scandinavian Plant Physiology Society.
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