Reactive oxygen species generated by infrared laser light in optical tweezers inhibits the germination of bacterial spores.
Bacillus
LTRS
ROS
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
revised:
28
04
2022
received:
25
03
2022
accepted:
08
05
2022
pubmed:
12
5
2022
medline:
5
8
2022
entrez:
11
5
2022
Statut:
ppublish
Résumé
Bacterial spores are highly resistant to heat, radiation and various disinfection chemicals. The impact of these on the biophysical and physicochemical properties of spores can be studied on the single-cell level using optical tweezers. However, the effect of the trapping laser on spores' germination rate is not fully understood. In this work, we assess the impact of 1064 nm laser light on the germination of Bacillus thuringiensis spores. The results show that the germination rate of spores after laser exposure follows a sigmoid dose-response relationship, with only 15% of spores germinating after 20 J of laser light. Under anaerobic growth conditions, the percentage of germinating spores at 20 J increased to 65%. The results thereby indicate that molecular oxygen is a major contributor to the germination-inhibiting effect observed. Thus, our study highlights the risk for optical trapping of spores and ways to mitigate it.
Identifiants
pubmed: 35538633
doi: 10.1002/jbio.202200081
doi:
Substances chimiques
Reactive Oxygen Species
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200081Subventions
Organisme : Kempestiftelserna
ID : JCK-1916.2
Organisme : Umeå University Industrial Doctoral School
Organisme : Vetenskapsrådet
ID : 2019-04016
Informations de copyright
© 2022 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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