Advances in antibody phage display technology.
Antibody discovery
Antigen presentation
Library design
Phage display
Selection strategy
Journal
Drug discovery today
ISSN: 1878-5832
Titre abrégé: Drug Discov Today
Pays: England
ID NLM: 9604391
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
received:
14
01
2022
revised:
24
03
2022
accepted:
04
05
2022
pubmed:
14
5
2022
medline:
22
6
2022
entrez:
13
5
2022
Statut:
ppublish
Résumé
Phage display technology can be used for the discovery of antibodies for research, diagnostic, and therapeutic purposes. In this review, we present and discuss key parameters that can be optimized when performing phage display selection campaigns, including the use of different antibody formats and advanced strategies for antigen presentation, such as immobilization, liposomes, nanodiscs, virus-like particles, and whole cells. Furthermore, we provide insights into selection strategies that can be used for the discovery of antibodies with complex binding requirements, such as targeting a specific epitope, cross-reactivity, or pH-dependent binding. Lastly, we provide a description of specialized phage display libraries for the discovery of bispecific antibodies and pH-sensitive antibodies. Together, these methods can be used to improve antibody discovery campaigns against all types of antigens.
Identifiants
pubmed: 35550436
pii: S1359-6446(22)00173-8
doi: 10.1016/j.drudis.2022.05.002
pii:
doi:
Substances chimiques
Antibodies
0
Epitopes
0
Peptide Library
0
Types de publication
Journal Article
Review
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2151-2169Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Ltd.. All rights reserved.