The conjugation of SUMO to the transcription factor MYC2 functions in blue light-mediated seedling development in Arabidopsis.
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
30 07 2022
30 07 2022
Historique:
received:
28
06
2021
accepted:
04
05
2022
pubmed:
15
5
2022
medline:
3
8
2022
entrez:
14
5
2022
Statut:
ppublish
Résumé
A key function of photoreceptor signaling is the coordinated regulation of a large number of genes to optimize plant growth and development. The basic helix loop helix (bHLH) transcription factor MYC2 is crucial for regulating gene expression in Arabidopsis thaliana during development in blue light. Here we demonstrate that blue light induces the SUMOylation of MYC2. Non-SUMOylatable MYC2 is less effective in suppressing blue light-mediated photomorphogenesis than wild-type (WT) MYC2. MYC2 interacts physically with the SUMO proteases SUMO PROTEASE RELATED TO FERTILITY1 (SPF1) and SPF2. Blue light exposure promotes the degradation of SPF1 and SPF2 and enhances the SUMOylation of MYC2. Phenotypic analysis revealed that SPF1/SPF2 function redundantly as positive regulators of blue light-mediated photomorphogenesis. Our data demonstrate that SUMO conjugation does not affect the dimerization of MYC transcription factors but modulates the interaction of MYC2 with its cognate DNA cis-element and with the ubiquitin ligase Plant U-box 10 (PUB10). Finally, we show that non-SUMOylatable MYC2 is less stable and interacts more strongly with PUB10 than the WT. Taken together, we conclude that SUMO functions as a counterpoint to the ubiquitin-mediated degradation of MYC2, thereby enhancing its function in blue light signaling.
Identifiants
pubmed: 35567527
pii: 6585902
doi: 10.1093/plcell/koac142
pmc: PMC9338799
doi:
Substances chimiques
Arabidopsis Proteins
0
Basic Helix-Loop-Helix Leucine Zipper Transcription Factors
0
Cyclopentanes
0
MYC2 protein, Arabidopsis
0
Transcription Factors
0
Ubiquitins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2892-2906Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/V003534/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T010533/1
Pays : United Kingdom
Informations de copyright
� The Author(s) 2022. Published by Oxford University Press on behalf of American Society of Plant Biologists.
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