A fit-less approach to the elasticity of the handles in optical tweezers experiments.
Data analysis
Optical tweezers
Journal
European biophysics journal : EBJ
ISSN: 1432-1017
Titre abrégé: Eur Biophys J
Pays: Germany
ID NLM: 8409413
Informations de publication
Date de publication:
Jul 2022
Jul 2022
Historique:
received:
22
02
2022
accepted:
02
05
2022
revised:
01
05
2022
pubmed:
23
5
2022
medline:
29
6
2022
entrez:
22
5
2022
Statut:
ppublish
Résumé
The elastic properties of the double-stranded DNA handles used in optical tweezers experiments on biomolecules are customarily modeled by an extensible worm-like chain model. Fitting such a model to experimental data, however, is no trivial task, as the function depends on four parameters in a highly non-linear fashion. We hereby propose a method to bypass the fitting procedure and obtain an empirical force vs. extension curve that accurately reproduces the elasticity of the handles.
Identifiants
pubmed: 35599262
doi: 10.1007/s00249-022-01603-2
pii: 10.1007/s00249-022-01603-2
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
413-418Subventions
Organisme : Università Degli Studi di Modena e Reggio Emila
ID : 020145_17_FDA_CARRAFAR2016INTER
Informations de copyright
© 2022. European Biophysical Societies' Association.
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