In vitro drought stress and drought-related gene expression in banana.
CDPK
Gene expression
Micropropagation
Plant tissue culture
qRT-PCR
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Jun 2022
Jun 2022
Historique:
received:
11
02
2022
accepted:
19
04
2022
pubmed:
27
5
2022
medline:
14
7
2022
entrez:
26
5
2022
Statut:
ppublish
Résumé
Banana is largely grown in tropical and subtropical climates. It is rich in various food components and has quite a high economic value. Unavailable ecological and agricultural conditions cause quantitative and qualitative losses in banana cultivation. Along with global climate change, drought stress is becoming prominent day by day. Micropropagation and rooting performance of Azman and Grand Naine banana cultivars were investigated under in vitro drought stress conditions. The expression levels of four different genes of CDPK gene family in leaf and root tissues of the rooted plants were determined with the use of qRT-PCR. Greater expression levels of four MaCDPK genes were seen in Azman cultivar than in Grand Naine cultivar. MaCDPK9 and MaCDPK21 had greater expression levels in root tissue and MaCDPK1 and MaCDPK40 genes in leaf tissues. Response of different banana cultivars to in vitro drought stress was determined in this study. The expression levels of the genes of CDPK gene family with a significant role in drought stress had significant contributions in elucidation of banana plant response to drought stress.
Sections du résumé
BACKGROUND
BACKGROUND
Banana is largely grown in tropical and subtropical climates. It is rich in various food components and has quite a high economic value. Unavailable ecological and agricultural conditions cause quantitative and qualitative losses in banana cultivation. Along with global climate change, drought stress is becoming prominent day by day.
METHODS AND RESULTS
RESULTS
Micropropagation and rooting performance of Azman and Grand Naine banana cultivars were investigated under in vitro drought stress conditions. The expression levels of four different genes of CDPK gene family in leaf and root tissues of the rooted plants were determined with the use of qRT-PCR. Greater expression levels of four MaCDPK genes were seen in Azman cultivar than in Grand Naine cultivar. MaCDPK9 and MaCDPK21 had greater expression levels in root tissue and MaCDPK1 and MaCDPK40 genes in leaf tissues.
CONCLUSIONS
CONCLUSIONS
Response of different banana cultivars to in vitro drought stress was determined in this study. The expression levels of the genes of CDPK gene family with a significant role in drought stress had significant contributions in elucidation of banana plant response to drought stress.
Identifiants
pubmed: 35616758
doi: 10.1007/s11033-022-07490-2
pii: 10.1007/s11033-022-07490-2
doi:
Substances chimiques
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5577-5583Subventions
Organisme : Çukurova Üniversitesi
ID : FYL-2021-13336
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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