Carbon dioxide fixation via production of succinic acid from glycerol in engineered Saccharomyces cerevisiae.

Bicarbonates / metabolism Carbon Dioxide / metabolism Culture Media / metabolism Glycerol / metabolism Glyoxylates / metabolism Saccharomyces cerevisiae / genetics Succinic Acid / metabolism
CO2 fixing Carbon dioxide Dicarboxylic acids Fermentation Glycerol Metabolic engineering Saccharomyces cerevisiae Succinic acid Yeast

Journal

Microbial cell factories
ISSN: 1475-2859
Titre abrégé: Microb Cell Fact
Pays: England
ID NLM: 101139812

Informations de publication

Date de publication:
28 May 2022
Historique:
received: 28 01 2022
accepted: 06 05 2022
entrez: 1 6 2022
pubmed: 2 6 2022
medline: 7 6 2022
Statut: epublish

Résumé

The microbial production of succinic acid (SA) from renewable carbon sources via the reverse TCA (rTCA) pathway is a process potentially accompanied by net-fixation of carbon dioxide (CO By changing the design of the expression cassettes for the rTCA pathway, overexpressing PYC2, and adding CaCO The data strongly suggest that a major part of dicarboxylic acids in our 2nd-generation SA-producer was formed via the rTCA pathway enabling a net fixation of CO

Sections du résumé

BACKGROUND BACKGROUND
The microbial production of succinic acid (SA) from renewable carbon sources via the reverse TCA (rTCA) pathway is a process potentially accompanied by net-fixation of carbon dioxide (CO
RESULTS RESULTS
By changing the design of the expression cassettes for the rTCA pathway, overexpressing PYC2, and adding CaCO
CONCLUSIONS CONCLUSIONS
The data strongly suggest that a major part of dicarboxylic acids in our 2nd-generation SA-producer was formed via the rTCA pathway enabling a net fixation of CO

Identifiants

DOI: 10.1186/s12934-022-01817-1 PMID: 35643577 PMC: PMC9148483
pubmed: 35643577
doi: 10.1186/s12934-022-01817-1
pii: 10.1186/s12934-022-01817-1
pmc: PMC9148483
doi:

Substances chimiques

Bicarbonates 0
Culture Media 0
Glyoxylates 0
Carbon Dioxide 142M471B3J
Succinic Acid AB6MNQ6J6L
Glycerol PDC6A3C0OX

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

102

Subventions

Organisme : Deutsche Forschungsgemeinschaft
ID : NE 697/7-1
Organisme : Horizon 2020 Framework Programme
ID : 764927

Informations de copyright

© 2022. The Author(s).

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Auteurs

Zahabiya Malubhoy (Z)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany.

Frederico Mendonça Bahia (FM)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany.
Dipartimento Di Biotecnologie E Bioscienze, Università Degli Studi Di Milano-Bicocca, Piazza della Scienza, 4, 67056, Milan, Italy.

Sophie Claire de Valk (SC)

Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

Erik de Hulster (E)

Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

Toni Rendulić (T)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany.

Juan Paulo Ragas Ortiz (JPR)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany.

Joeline Xiberras (J)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany.
RB/WD - A030, BASF SE, Carl-Bosch-Strasse 38, 67056, Ludwigshafen am Rhein, Germany.

Mathias Klein (M)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany.

Robert Mans (R)

Department of Biotechnology, Delft University of Technology, van der Maasweg 9, 2629 HZ, Delft, The Netherlands.

Elke Nevoigt (E)

Department of Life Sciences and Chemistry, Jacobs University Bremen gGmbH, Campus Ring 1, 28759, Bremen, Germany. e.nevoigt@jacobs-university.de.

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Classifications MeSH

questionsmedicales.fr › Produits chimiques inorganiques › Électrolytes › Ions › Anions › Carbonates › Hydrogénocarbonates › Carbon dioxide fixation via production of...
questionsmedicales.fr › Produits chimiques inorganiques › Composés de l'oxygène › Oxydes › Dioxyde de carbone › Carbon dioxide fixation via production of...
questionsmedicales.fr › Équipement et fournitures › Milieux de culture › Carbon dioxide fixation via production of...
questionsmedicales.fr › Glucides › Polyols › Dérivés du glycérol › Glycérol › Carbon dioxide fixation via production of...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides aldéhydiques › Glyoxylates › Carbon dioxide fixation via production of...
questionsmedicales.fr › Eucaryotes › Champignons › Levures › Saccharomyces › Saccharomyces cerevisiae › Carbon dioxide fixation via production of...
questionsmedicales.fr › Composés chimiques organiques › Acides carboxyliques › Acides acycliques › Diacides carboxyliques › Succinates › Acide succinique › Carbon dioxide fixation via production of...