An Argonaute from

None 5′-modified guide Argonaute endonuclease Thermus parvatiensis

Journal

Acta biochimica et biophysica Sinica
ISSN: 1745-7270
Titre abrégé: Acta Biochim Biophys Sin (Shanghai)
Pays: China
ID NLM: 101206716

Informations de publication

Date de publication:
25 05 2022
Historique:
pubmed: 2 6 2022
medline: 17 1 2023
entrez: 1 6 2022
Statut: ppublish

Résumé

Prokaryotic Argonaute (pAgo) nucleases with precise DNA cleavage activity show great potential for gene manipulation. Extensive biochemical studies have revealed that recognition of guides with different 5' groups by Ago is important for biocatalysis. Here, we identified an Ago from the thermophilic

Identifiants

pubmed: 35643958
doi: 10.3724/abbs.2022047
pmc: PMC9828299
doi:

Substances chimiques

DNA 9007-49-2
DNA, Single-Stranded 0
Endonucleases EC 3.1.-
Argonaute Proteins 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

686-695

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Auteurs

Yingying Sun (Y)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Xiang Guo (X)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Hui Lu (H)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Liuqing Chen (L)

Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China.

Fei Huang (F)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Qian Liu (Q)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

Yan Feng (Y)

State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China.

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Classifications MeSH