Reprint of: Generation of Superoxide Radical during Autoxidation of Hydroxylamine and an Assay for Superoxide Dismutase.
Journal
Archives of biochemistry and biophysics
ISSN: 1096-0384
Titre abrégé: Arch Biochem Biophys
Pays: United States
ID NLM: 0372430
Informations de publication
Date de publication:
15 09 2022
15 09 2022
Historique:
pubmed:
7
6
2022
medline:
20
7
2022
entrez:
6
6
2022
Statut:
ppublish
Résumé
Accompanying the autoxidation of hydroxylamine at pH 10.2, nitroblue tetrazolium was reduced and nitrite was produced in the presence of EDTA. The rate of autoxidation was negligible below pH 8.0, but sharply increased with increasing pH. The reduction of nitroblue tetrazolium was inhibited by superoxide dismutase, indicating the participation of superoxide anion radical in the autoxidation. Hydrogen peroxide stimulated the autoxidation and superoxide dismutase inhibited the hydrogen peroxide-induced oxidation, results which suggest the participation of hydrogen peroxide in autoxidation and in the generation of superoxide radical. An assay for superoxide dismutase using autoxidation of hydroxylamine is described.
Identifiants
pubmed: 35667906
pii: S0003-9861(22)00132-1
doi: 10.1016/j.abb.2022.109247
pii:
doi:
Substances chimiques
Hydroxylamines
0
Superoxides
11062-77-4
Nitroblue Tetrazolium
298-83-9
Hydroxylamine
2FP81O2L9Z
Hydrogen Peroxide
BBX060AN9V
Superoxide Dismutase
EC 1.15.1.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
109247Informations de copyright
Copyright © 2022. Published by Elsevier Inc.