Construction of Strong Promoters by Assembling Sigma Factor Binding Motifs.

Bacillus subtilis / genetics Base Sequence DNA-Directed RNA Polymerases / metabolism Escherichia coli / genetics Promoter Regions, Genetic Sigma Factor / genetics
Bacteria Library Sigma factor Stress-responsive Strong promoter Synthetic biology

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 21 6 2022
pubmed: 22 6 2022
medline: 24 6 2022
Statut: ppublish

Résumé

Development of strong promoters is of growing interest in the field of biotechnology and synthetic biology. Here we present a protocol for the construction of strong prokaryotic promoters that can be recognized by designated multiple sigma factors by interlocking their cognate binding motifs on DNA strands. Strong and stress responsive promoters for Escherichia coli and Bacillus subtilis have been created following the presented protocol. Customized promoters could be easily developed for fine-tuning gene expression or overproducing enzymes with prokaryotic cell factories.

Identifiants

DOI: 10.1007/978-1-0716-2152-3_9 PMID: 35727448
pubmed: 35727448
doi: 10.1007/978-1-0716-2152-3_9
doi:

Substances chimiques

Sigma Factor 0
DNA-Directed RNA Polymerases EC 2.7.7.6

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

137-147

Informations de copyright

© 2022. Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Yonglin Zhang (Y)

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.

Yang Wang (Y)

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.

Jianghua Li (J)

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Wuxi, China.

Chao Wang (C)

The Science Center for Future Foods, Jiangnan University, Wuxi, China.

Guocheng Du (G)

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Wuxi, China.

Zhen Kang (Z)

The Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, Jiangnan University, Wuxi, China. zkang@jiangnan.edu.cn.
The Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Wuxi, China. zkang@jiangnan.edu.cn.
The Science Center for Future Foods, Jiangnan University, Wuxi, China. zkang@jiangnan.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets sporulés à Gram positif › Bacillaceae › Bacillus › Bacillus subtilis › Construction of Strong Promoters by Assembling...
questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence nucléotidique › Construction of Strong Promoters by Assembling...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Phosphotransferases › Nucleotidyltransferases › RNA nucleotidyltransferases › DNA-directed RNA polymerases › Construction of Strong Promoters by Assembling...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Enterobacteriaceae › Escherichia › Escherichia coli › Construction of Strong Promoters by Assembling...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Gènes › Composants de gène › Éléments de régulation transcriptionnelle › Régions promotrices (génétique) › Construction of Strong Promoters by Assembling...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Facteurs de transcription › Facteur sigma › Construction of Strong Promoters by Assembling...