Serine/Threonine Ligation and Cysteine/Penicillamine Ligation.
Acidolysis
Chemical protein synthesis
Cyclic peptide
Cysteine/penicillamine ligation
Peptide salicylaldehyde ester
Serine/threonine ligation
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2022
2022
Historique:
entrez:
27
6
2022
pubmed:
28
6
2022
medline:
30
6
2022
Statut:
ppublish
Résumé
Serine/threonine ligation (STL) and cysteine/penicillamine ligation (CPL) are highly chemo- and regioselective reactions between unprotected peptides with C-terminus salicylaldehyde esters and unprotected peptides with N-terminus serine/threonine or cysteine/penicillamine, which serve as powerful tools for cyclic peptide natural product and chemical protein synthesis. Herein, we introduce the preparation of C-terminal peptide salicylaldehyde esters, serine/threonine ligation, cysteine/penicillamine ligation, and subsequent acidolysis.
Identifiants
pubmed: 35761040
doi: 10.1007/978-1-0716-2489-0_3
doi:
Substances chimiques
Esters
0
Peptides
0
Threonine
2ZD004190S
Serine
452VLY9402
Penicillamine
GNN1DV99GX
Cysteine
K848JZ4886
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-43Informations de copyright
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Références
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