Ocimum sanctum, OscWRKY1, regulates phenylpropanoid pathway genes and promotes resistance to pathogen infection in Arabidopsis.
Arabidopsis
/ metabolism
Arabidopsis Proteins
/ metabolism
Cinnamates
Depsides
Digoxigenin
/ metabolism
Disease Resistance
/ genetics
Gene Expression Regulation, Plant
Ocimum sanctum
/ metabolism
Plant Diseases
/ genetics
Pseudomonas syringae
/ physiology
Salicylic Acid
/ metabolism
Transcription Factors
/ genetics
Rosmarinic Acid
PAL genes
Phenylpropanoid
Pst DC3000
Rosmarinic acid
VIGS
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
Oct 2022
Oct 2022
Historique:
received:
04
02
2022
accepted:
18
06
2022
pubmed:
3
7
2022
medline:
12
10
2022
entrez:
2
7
2022
Statut:
ppublish
Résumé
OscWRKY1 from Ocimum sanctum positively regulates phenylpropanoid pathway genes and rosmarinic acid content. OscWRKY1 overexpression promotes resistance against bacterial pathogen in Arabidopsis. WRKY transcription factor (TF) family regulates various developmental and physiological functions in plants. PAL genes encode enzymes which are involved in plant defense responses, but the direct regulation of PAL genes and phenylpropanoid pathway through WRKY TF's is not well characterized. In the present study, we have characterized an OscWRKY1 gene from Ocimum sanctum which shows induced expression by methyl jasmonate (MeJA), salicylic acid (SA), and wounding. The recombinant OscWRKY1 protein binds to the DIG-labeled (Digoxigenin) W-box cis-element TTGAC[C/T] and activates the LacZ reporter gene in yeast. Overexpression of OscWRKY1 enhances Arabidopsis resistance towards Pseudomonas syringae pv. tomato Pst DC3000. Upstream activator sequences of PAL and C4H have been identified to contain the conserved W-box cis-element (TTGACC) in both O. sanctum and Arabidopsis. OscWRKY1 was found to interact with W-box cis-element present in the PAL and C4H promoters. Silencing of OscWRKY1 using VIGS resulted in reduced expression of PAL, C4H, COMT, F5H and 4CL transcripts. OscWRKY1 silenced plants exhibit reduced PAL activity, whereas, the overexpression lines of OscWRKY1 in Arabidopsis exhibit increased PAL activity. Furthermore, the metabolite analysis of OscWRKY1 silenced plants showed reduced rosmarinic acid content. These results revealed that OscWRKY1 positively regulates the phenylpropanoid pathway genes leading to the alteration of rosmarinic acid content and enhances the resistance against bacterial pathogen in Arabidopsis.
Identifiants
pubmed: 35780285
doi: 10.1007/s11103-022-01297-2
pii: 10.1007/s11103-022-01297-2
doi:
Substances chimiques
Arabidopsis Proteins
0
Cinnamates
0
Depsides
0
Transcription Factors
0
Digoxigenin
NQ1SX9LNAU
Salicylic Acid
O414PZ4LPZ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
235-251Subventions
Organisme : Central Institute of Medicinal and Aromatic Plants
ID : BSC203
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Nature B.V.
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