Fluid dynamics characterisation of a rotating bioreactor for tissue engineering.
Esophageal substitute
Rotating bioreactor
Spiral Poiseuille flow
Tissue engineering
Journal
Medical engineering & physics
ISSN: 1873-4030
Titre abrégé: Med Eng Phys
Pays: England
ID NLM: 9422753
Informations de publication
Date de publication:
07 2022
07 2022
Historique:
received:
11
10
2021
revised:
02
05
2022
accepted:
06
06
2022
entrez:
5
7
2022
pubmed:
6
7
2022
medline:
8
7
2022
Statut:
ppublish
Résumé
Biological scaffolds composed of extracellular matrix (ECM) derived from decellularised tissue are increasingly used in regenerative medicine. In this project, a flow perfusion bioreactor (the rotary cell culture system (RCCS), commercially available from Synthecon (Houston, TX)) is used in order to obtain some esophageal extracellular matrix. A theoretical mechanical characterisation of this experimental set-up is provided. Due to the combination of rotation and perfusion, some spiral Poiseuille flow is created inside the tubular esophagus. In a transverse section, a particle (or cell) experiences simultaneously gravitational, Archimedes, centrifugal, Coriolis, and drag forces. In a frame of reference rotating with angular velocity ω, the particle follows a periodic nearly circular path in the clockwise direction, associated with a very slow centrifugal drift towards the esophagus wall. It appears that moderate perfusion rate and rotation speed (ω < 20 rpm and Q < 30 ml/min) are appropriate experimental conditions for esophagus tissue engineering using the RCCS Synthecon bioreactor.
Identifiants
pubmed: 35781390
pii: S1350-4533(22)00080-7
doi: 10.1016/j.medengphy.2022.103831
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103831Informations de copyright
Copyright © 2022 IPEM. Published by Elsevier Ltd. All rights reserved.