Ovule siRNAs methylate protein-coding genes in trans.
Journal
The Plant cell
ISSN: 1532-298X
Titre abrégé: Plant Cell
Pays: England
ID NLM: 9208688
Informations de publication
Date de publication:
27 09 2022
27 09 2022
Historique:
received:
14
06
2021
accepted:
24
06
2022
pubmed:
6
7
2022
medline:
30
9
2022
entrez:
5
7
2022
Statut:
ppublish
Résumé
Twenty-four-nucleotide (nt) small interfering RNAs (siRNAs) maintain asymmetric DNA methylation at thousands of euchromatic transposable elements in plant genomes in a process called RNA-directed DNA methylation (RdDM). RdDM is dispensable for growth and development in Arabidopsis thaliana, but is required for reproduction in other plants, such as Brassica rapa. The 24-nt siRNAs are abundant in maternal reproductive tissue, due largely to overwhelming expression from a few loci in the ovule and developing seed coat, termed siren loci. A recent study showed that 24-nt siRNAs produced in the anther tapetal tissue can methylate male meiocyte genes in trans. Here we show that in B. rapa, a similar process takes place in female tissue. siRNAs are produced from gene fragments embedded in some siren loci, and these siRNAs can trigger methylation in trans at related protein-coding genes. This trans-methylation is associated with silencing of some target genes and may be responsible for seed abortion in RdDM mutants. Furthermore, we demonstrate that a consensus sequence in at least two families of DNA transposons is associated with abundant siren expression, most likely through recruitment of CLASSY3, a putative chromatin remodeler. This research describes a mechanism whereby RdDM influences gene expression and sheds light on the role of RdDM during plant reproduction.
Identifiants
pubmed: 35781738
pii: 6628591
doi: 10.1093/plcell/koac197
pmc: PMC9516104
doi:
Substances chimiques
Arabidopsis Proteins
0
Chromatin
0
DNA Transposable Elements
0
Nucleotides
0
RNA, Plant
0
RNA, Small Interfering
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3647-3664Commentaires et corrections
Type : CommentIn
Informations de copyright
© American Society of Plant Biologists 2022. All rights reserved. For permissions, please email: journals.permissions@oup.com.
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