Polaramycin B, and not physical interaction, is the signal that rewires fungal metabolism in the Streptomyces-Aspergillus interaction.
Journal
Environmental microbiology
ISSN: 1462-2920
Titre abrégé: Environ Microbiol
Pays: England
ID NLM: 100883692
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
received:
10
05
2022
accepted:
26
06
2022
pubmed:
19
7
2022
medline:
20
10
2022
entrez:
18
7
2022
Statut:
ppublish
Résumé
Co-culturing the bacterium Streptomyces rapamycinicus and the ascomycete Aspergillus nidulans has previously been shown to trigger the production of orsellinic acid (ORS) and its derivates in the fungal cells. Based on these studies it was assumed that direct physical contact is a prerequisite for the metabolic reaction that involves a fungal amino acid starvation response and activating chromatin modifications at the biosynthetic gene cluster (BGC). Here we show that not physical contact, but a guanidine containing macrolide, named polaramycin B, triggers the response. The substance is produced constitutively by the bacterium and above a certain concentration, provokes the production of ORS. In addition, several other secondary metabolites were induced by polaramycin B. Our genome-wide transcriptome analysis showed that polaramycin B treatment causes downregulation of fungal genes necessary for membrane stability, general metabolism and growth. A compensatory genetic response can be observed in the fungus that included upregulation of BGCs and genes necessary for ribosome biogenesis, translation and membrane stability. Our work discovered a novel chemical communication, in which the antifungal bacterial metabolite polaramycin B leads to the production of antibacterial defence chemicals and to the upregulation of genes necessary to compensate for the cellular damage caused by polaramycin B.
Identifiants
pubmed: 35848075
doi: 10.1111/1462-2920.16118
pmc: PMC9796313
doi:
Substances chimiques
Amino Acids
0
Anti-Bacterial Agents
0
Antifungal Agents
0
Chromatin
0
Electrolytes
0
Guanidines
0
Macrolides
0
orsellinic acid
11XLA0494B
polaramycin B
0
Resorcinols
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
4899-4914Informations de copyright
© 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd.
Références
Bioinformatics. 2015 Jan 15;31(2):166-9
pubmed: 25260700
Fungal Genet Biol. 2011 Jan;48(1):62-9
pubmed: 20659575
Mol Biosyst. 2010 Mar;6(3):587-93
pubmed: 20174687
Fungal Genet Biol. 2019 Apr;125:71-83
pubmed: 30731202
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
Toxins (Basel). 2016 Nov 28;8(12):
pubmed: 27916804
Front Microbiol. 2019 Aug 13;10:1759
pubmed: 31456754
PLoS Genet. 2012 Sep;8(9):e1002952
pubmed: 23028359
Front Microbiol. 2016 Jul 26;7:1113
pubmed: 27507961
BMC Bioinformatics. 2016 Oct 21;17(1):428
pubmed: 27769170
FEMS Microbiol Rev. 2005 Sep;29(4):795-811
pubmed: 16102603
Biotechnol Adv. 2019 Nov 1;37(6):107345
pubmed: 30738111
Molecules. 2016 May 10;21(5):
pubmed: 27171072
Bioinformatics. 2009 Jul 15;25(14):1754-60
pubmed: 19451168
Curr Opin Microbiol. 2017 Jun;37:135-141
pubmed: 28692866
Microbiol Spectr. 2017 Jan;5(1):
pubmed: 28155813
Proc Natl Acad Sci U S A. 2011 Aug 23;108(34):14282-7
pubmed: 21825172
Metabolites. 2012 Jan 30;2(1):100-33
pubmed: 24957370
Mol Microbiol. 2001 Sep;41(5):1077-89
pubmed: 11555288
Nat Rev Microbiol. 2005 Dec;3(12):937-47
pubmed: 16322742
Elife. 2018 Oct 12;7:
pubmed: 30311911
Nat Genet. 2000 May;25(1):25-9
pubmed: 10802651
Environ Microbiol. 2022 Oct;24(10):4899-4914
pubmed: 35848075
Proc Natl Acad Sci U S A. 2009 Aug 25;106(34):14558-63
pubmed: 19666480
Nat Chem Biol. 2009 Jul;5(7):462-4
pubmed: 19448638
Molecules. 2019 Oct 30;24(21):
pubmed: 31671653
Adv Genet. 1953;5:141-238
pubmed: 13040135
Nucleic Acids Res. 2011 Jul;39(Web Server issue):W339-46
pubmed: 21672958
J Antibiot (Tokyo). 1967 Mar;20(2):93-108
pubmed: 4861279
Genome Biol. 2014 Feb 03;15(2):R29
pubmed: 24485249
ACS Chem Biol. 2016 Aug 19;11(8):2117-23
pubmed: 27310134
Fungal Genet Biol. 2016 Apr;89:18-28
pubmed: 26808821
Mol Biol Cell. 1997 Dec;8(12):2421-36
pubmed: 9398665
FEMS Microbiol Ecol. 2007 Dec;62(3):258-67
pubmed: 17991019
Mol Microbiol. 2000 Nov;38(3):658-65
pubmed: 11069688
Front Plant Sci. 2015 Aug 06;6:573
pubmed: 26300892
Microbiol Rev. 1993 Sep;57(3):595-604
pubmed: 8246841
Annu Rev Phytopathol. 2005;43:437-58
pubmed: 16078891
BMC Plant Biol. 2011 Jul 28;11:110
pubmed: 21798058
Yao Xue Xue Bao. 1997 May;32(5):352-6
pubmed: 11498870
Nat Rev Microbiol. 2013 Jan;11(1):21-32
pubmed: 23178386
Anal Bioanal Chem. 2020 Apr;412(11):2607-2620
pubmed: 32078002
PLoS Genet. 2016 Aug 22;12(8):e1006222
pubmed: 27548260
Fungal Genet Biol. 2007 Nov;44(11):1134-45
pubmed: 17291795
ACS Chem Biol. 2012 Mar 16;7(3):599-606
pubmed: 22234162
Appl Microbiol Biotechnol. 2012 Sep;95(6):1389-404
pubmed: 22814413
Med Mycol. 2006 Sep 1;44(Supplement_1):S91-S94
pubmed: 30408941
Annu Rev Genet. 2016 Nov 23;50:371-392
pubmed: 27732794