Purple acid phosphatase2 stimulates a futile cycle of lipid synthesis and degradation, and mitigates the negative growth effects of triacylglycerol accumulation in vegetative tissues.
Arabidopsis
/ metabolism
Arabidopsis Proteins
/ metabolism
Carbon
/ metabolism
Carboxylic Ester Hydrolases
Diglycerides
/ metabolism
Fatty Acids
/ metabolism
Gene Expression Regulation, Plant
Lipase
/ metabolism
Membrane Lipids
/ metabolism
Phospholipids
/ metabolism
Plant Oils
/ metabolism
Plants, Genetically Modified
/ metabolism
Seeds
/ metabolism
Substrate Cycling
Sugars
/ metabolism
Transcription Factors
Triglycerides
/ metabolism
Arabidopsis thaliana
biomass
carbon allocation
carbon assimilation
lipid metabolism
triacylglycerol
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
11 2022
11 2022
Historique:
received:
12
04
2022
accepted:
07
07
2022
pubmed:
20
7
2022
medline:
12
10
2022
entrez:
19
7
2022
Statut:
ppublish
Résumé
Storage lipids (mostly triacylglycerols, TAGs) serve as an important energy and carbon reserve in plants, and hyperaccumulation of TAG in vegetative tissues can have negative effects on plant growth. Purple acid phosphatase2 (PAP2) was previously shown to affect carbon metabolism and boost plant growth. However, the effects of PAP2 on lipid metabolism remain unknown. Here, we demonstrated that PAP2 can stimulate a futile cycle of fatty acid (FA) synthesis and degradation, and mitigate negative growth effects associated with high accumulation of TAG in vegetative tissues. Constitutive expression of PAP2 in Arabidopsis thaliana enhanced both lipid synthesis and degradation in leaves and led to a substantial increase in seed oil yield. Suppressing lipid degradation in a PAP2-overexpressing line by disrupting sugar-dependent1 (SDP1), a predominant TAG lipase, significantly elevated vegetative TAG content and improved plant growth. Diverting FAs from membrane lipids to TAGs in PAP2-overexpressing plants by constitutively expressing phospholipid:diacylglycerol acyltransferase1 (PDAT1) greatly increased TAG content in vegetative tissues without compromising biomass yield. These results highlight the potential of combining PAP2 with TAG-promoting factors to enhance carbon assimilation, FA synthesis and allocation to TAGs for optimized plant growth and storage lipid accumulation in vegetative tissues.
Substances chimiques
Arabidopsis Proteins
0
Diglycerides
0
Fatty Acids
0
Membrane Lipids
0
PAP2 protein, Arabidopsis
0
Phospholipids
0
Plant Oils
0
Sugars
0
Transcription Factors
0
Triglycerides
0
Carbon
7440-44-0
Carboxylic Ester Hydrolases
EC 3.1.1.-
Lipase
EC 3.1.1.3
SDP1 protein, Arabidopsis
EC 3.1.1.3
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1128-1139Informations de copyright
© 2022 No claim to US Government works New Phytologist © 2022 New Phytologist Foundation.
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