A CRISPR screen targeting PI3K effectors identifies RASA3 as a negative regulator of LFA-1-mediated adhesion in T cells.

Animals Antigens, CD Cell Adhesion / genetics Cell Adhesion Molecules Clustered Regularly Interspaced Short Palindromic Repeats GTPase-Activating Proteins Intercellular Adhesion Molecule-1 / metabolism Lymphocyte Function-Associated Antigen-1 / genetics Mice Phosphatidylinositol 3-Kinase / metabolism Phosphatidylinositol 3-Kinases / genetics T-Lymphocytes / metabolism

Journal

Science signaling
ISSN: 1937-9145
Titre abrégé: Sci Signal
Pays: United States
ID NLM: 101465400

Informations de publication

Date de publication:
19 07 2022
Historique:
entrez: 20 7 2022
pubmed: 21 7 2022
medline: 23 7 2022
Statut: ppublish

Résumé

The integrin lymphocyte function-associated antigen 1 (LFA-1) helps to coordinate the migration, adhesion, and activation of T cells through interactions with intercellular adhesion molecule 1 (ICAM-1) and ICAM-2. LFA-1 is activated during the engagement of chemokine receptors and the T cell receptor (TCR) through inside-out signaling, a process that is partially mediated by phosphoinositide 3-kinase (PI3K) and its product phosphatidylinositol 3,4,5-trisphosphate (PIP

Identifiants

DOI: 10.1126/scisignal.abl9169 PMID: 35857633 PMC: PMC9637254
pubmed: 35857633
doi: 10.1126/scisignal.abl9169
pmc: PMC9637254
mid: NIHMS1831271
doi:

Substances chimiques

Antigens, CD 0
Cell Adhesion Molecules 0
GTPase-Activating Proteins 0
ICAM-2 protein, mouse 0
Lymphocyte Function-Associated Antigen-1 0
RASA3 protein, mouse 0
Intercellular Adhesion Molecule-1 126547-89-5
Phosphatidylinositol 3-Kinase EC 2.7.1.137

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, N.I.H., Intramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

eabl9169

Subventions

Organisme : British Heart Foundation
ID : FS/20/23/34784
Pays : United Kingdom
Organisme : NHLBI NIH HHS
ID : R35 HL144976
Pays : United States
Organisme : Wellcome Trust
ID : 200925/Z/16/Z
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/T007826/1
Pays : United Kingdom

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Auteurs

Kristoffer H Johansen (KH)

Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
Section of Experimental and Translational Immunology, Department of Health Technology, Technical University of Denmark, Kongens Lyngby DK-2800, Denmark.
ORCID: 0000-0002-7711-9451

Dominic P Golec (DP)

Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
ORCID: 0000-0002-2741-3302

Bonnie Huang (B)

Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
ORCID: 0000-0001-5931-6075

Chung Park (C)

B-Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
ORCID: 0000-0002-7819-5333

Julie H Thomsen (JH)

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
ORCID: 0000-0002-0091-3614

Silvia Preite (S)

Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Jennifer L Cannons (JL)

Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Fabien Garçon (F)

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.

Edward C Schrom (EC)

Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
ORCID: 0000-0002-1793-6433

Christina J F Courrèges (CJF)

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
ORCID: 0000-0003-0203-8486

Tibor Z Veres (TZ)

Lymphocyte Biology Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
ORCID: 0000-0002-6980-8900

James Harrison (J)

Cardiovascular Division, Department of Medicine, University of Cambridge, Cambridge CB2 0QQ, UK.
ORCID: 0000-0003-4960-5062

Meritxell Nus (M)

Cardiovascular Division, Department of Medicine, University of Cambridge, Cambridge CB2 0QQ, UK.
ORCID: 0000-0002-6378-8910

James D Phelan (JD)

Lymphoid Malignancies Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA.

Wolfgang Bergmeier (W)

Biochemistry and Biophysics, School of Medicine, University of North Carolina, Chapel Hill, NC 27599, USA.
ORCID: 0000-0002-1211-8861

John H Kehrl (JH)

B-Cell Molecular Immunology Section, Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.

Klaus Okkenhaug (K)

Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, UK.
ORCID: 0000-0002-9432-4051

Pamela L Schwartzberg (PL)

Cell Signaling and Immunity Section, Laboratory of Immune System Biology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
National Human Genome Research Institute, National Institutes of Health, Bethesda, MD 20892, USA.
ORCID: 0000-0001-6318-9187

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Facteurs biologiques › Marqueurs biologiques › Antigènes de différenciation › Antigènes CD › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Adhérence cellulaire › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes de surface › Molécules d'adhérence cellulaire › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Génome › Composants de génome › Séquences répétées en tandem › Clustered regularly interspaced short palindromic repeats › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Régulateurs des protéines G › Protéines d'activation de la GTPase › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes de surface › Molécules d'adhérence cellulaire › Molécule-1 d'adhérence intercellulaire › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Facteurs biologiques › Antigènes › Antigènes de surface › Molécules d'adhérence cellulaire › Récepteurs d'écotaxie des lymphocytes › Antigène-1 associé à la fonction du lymphocyte › A CRISPR screen targeting PI3K effectors...
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questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphatidylinositol 3-kinases › Phosphatidylinositol 3-kinase › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines et peptides de signalisation intracellulaire › Phosphatidylinositol 3-kinases › A CRISPR screen targeting PI3K effectors...
questionsmedicales.fr › Systèmes sanguin et immunitaire › Système immunitaire › Leucocytes › Agranulocytes › Lymphocytes › Lymphocytes T › A CRISPR screen targeting PI3K effectors...