Building biomaterials through genetic code expansion.

Biocompatible Materials Genetic Code Proteins / genetics Genetic Engineering Amino Acids / metabolism Amines
bioinspired biomaterials engineered protein genetic code expansion tunable biomaterials unnatural amino acid

Journal

Trends in biotechnology
ISSN: 1879-3096
Titre abrégé: Trends Biotechnol
Pays: England
ID NLM: 8310903

Informations de publication

Date de publication:
02 2023
Historique:
received: 10 02 2022
revised: 30 06 2022
accepted: 08 07 2022
pubmed: 1 8 2022
medline: 24 1 2023
entrez: 31 7 2022
Statut: ppublish

Résumé

Genetic code expansion (GCE) enables directed incorporation of noncoded amino acids (NCAAs) and unnatural amino acids (UNAAs) into the active core that confers dedicated structure and function to engineered proteins. Many protein biomaterials are tandem repeats that intrinsically include NCAAs generated through post-translational modifications (PTMs) to execute assigned functions. Conventional genetic engineering approaches using prokaryotic systems have limited ability to biosynthesize functionally active biomaterials with NCAAs/UNAAs. Codon suppression and reassignment introduce NCAAs/UNAAs globally, allowing engineered proteins to be redesigned to mimic natural matrix-cell interactions for tissue engineering. Expanding the genetic code enables the engineering of biomaterials with catechols - growth factor mimetics that modulate cell-matrix interactions - thereby facilitating tissue-specific expression of genes and proteins. This method of protein engineering shows promise in achieving tissue-informed, tissue-compliant tunable biomaterials.

Identifiants

DOI: 10.1016/j.tibtech.2022.07.003 PMID: 35908989
pubmed: 35908989
pii: S0167-7799(22)00172-X
doi: 10.1016/j.tibtech.2022.07.003
pii:
doi:

Substances chimiques

Biocompatible Materials 0
Proteins 0
Amino Acids 0
Amines 0

Types de publication

Journal Article Review Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

165-183

Informations de copyright

Copyright © 2022 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests The authors declare no conflicts of interest.

Auteurs

Valappil Sisila (V)

Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) Central Leather Research Institute (CLRI), Chennai, Tamil Nadu 600020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India.

Mohan Indhu (M)

Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) Central Leather Research Institute (CLRI), Chennai, Tamil Nadu 600020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India.

Janani Radhakrishnan (J)

Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) Central Leather Research Institute (CLRI), Chennai, Tamil Nadu 600020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India. Electronic address: janani@clri.res.in.

Niraikulam Ayyadurai (N)

Department of Biochemistry and Biotechnology, Council of Scientific and Industrial Research (CSIR) Central Leather Research Institute (CLRI), Chennai, Tamil Nadu 600020, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201 002, India. Electronic address: ayyadurai@clri.res.in.

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Classifications MeSH

questionsmedicales.fr › Technologie, industrie et agriculture › Produits manufacturés › Matériaux biomédicaux et dentaires › Matériaux biocompatibles › Building biomaterials through genetic code expansion.
questionsmedicales.fr › Phénomènes génétiques › Structures génétiques › Code génétique › Building biomaterials through genetic code expansion.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Building biomaterials through genetic code expansion.
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Génie génétique › Building biomaterials through genetic code expansion.
questionsmedicales.fr › Acides aminés, peptides et protéines › Acides aminés › Building biomaterials through genetic code expansion.
questionsmedicales.fr › Composés chimiques organiques › Amines › Building biomaterials through genetic code expansion.