The Fluorescent Enzyme Cascade Detects Low Abundance Protein Modifications Suitable for the Assembly of Functionally Annotated Modificatome Databases.
analytical methods
conformation analysis
enzymes
functional annotation
protein modifications
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
06 10 2022
06 10 2022
Historique:
revised:
27
07
2022
received:
14
07
2022
pubmed:
4
8
2022
medline:
12
10
2022
entrez:
3
8
2022
Statut:
ppublish
Résumé
Pathophysiological functions of proteins critically depend on both their chemical composition, including post-translational modifications, and their three-dimensional structure, commonly referred to as structure-activity relationship. Current analytical methods, like capillary electrophoresis or mass spectrometry, suffer from limitations, such as the detection of unexpected modifications at low abundance and their insensitivity to conformational changes. Building on previous enzyme-based analytical methods, we here introduce a fluorescence-based enzyme cascade (fEC), which can detect diverse chemical and conformational variations in protein samples and assemble them into digital databases. Together with complementary analytical methods an automated fEC analysis established unique modification-function relationships, which can be expanded to a proteome-wide scale, i. e. a functionally annotated modificatome. The fEC offers diverse applications, including hypersensitive biomarker detection in complex samples.
Identifiants
pubmed: 35920326
doi: 10.1002/cbic.202200399
doi:
Substances chimiques
Proteome
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200399Informations de copyright
© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.
Références
Curr Protoc Chem Biol. 2011;3(4):153-162
pubmed: 22844652
Nature. 2012 Mar 28;483(7391):531-3
pubmed: 22460880
Anal Chem. 2018 Apr 17;90(8):5055-5065
pubmed: 29582994
Drug Discov Today. 2010 Sep;15(17-18):773-80
pubmed: 20599624
Nanoscale. 2021 Dec 16;13(48):20508-20520
pubmed: 34854455
Biotechniques. 2019 Sep;67(3):126-137
pubmed: 31379198
Genes Dev. 1997 Apr 1;11(7):815-23
pubmed: 9106654
Biol Chem. 2020 Oct 25;401(11):1233-1248
pubmed: 32745066
Trends Cell Biol. 2000 Aug;10(8):322-8
pubmed: 10884684
Nat Rev Drug Discov. 2012 Jun 29;11(7):527-40
pubmed: 22743980
Arch Immunol Ther Exp (Warsz). 2012 Oct;60(5):331-44
pubmed: 22930363
Chembiochem. 2022 Oct 6;23(19):e202200399
pubmed: 35920326
Nature. 2016 May 25;533(7604):452-4
pubmed: 27225100
Expert Rev Proteomics. 2018 May;15(5):431-449
pubmed: 29694790
Biotechnol Prog. 2012 May-Jun;28(3):608-22
pubmed: 22473974
Nature. 2021 Aug;596(7873):583-589
pubmed: 34265844
J Pharm Sci. 2016 Feb;105(2):460-475
pubmed: 26869412
Biol Chem. 2018 Sep 25;399(9):1009-1022
pubmed: 29975661
Chem Rev. 2022 Apr 27;122(8):7269-7326
pubmed: 34415162
J Pharm Biomed Anal. 2020 Jul 15;186:113251
pubmed: 32251978
Adv Drug Deliv Rev. 2013 Oct;65(10):1357-69
pubmed: 23026637
Ther Adv Drug Saf. 2011 Jun;2(3):113-28
pubmed: 25083207
Biomolecules. 2021 Jan 04;11(1):
pubmed: 33406777
Appl Microbiol Biotechnol. 2004 May;64(4):447-54
pubmed: 14740191
Acta Pharmacol Sin. 2021 Jul;42(7):1015-1017
pubmed: 33087837
Nat Rev Drug Discov. 2011 Aug 31;10(9):712
pubmed: 21892149