A novel WRKY34-bZIP3 module regulates phenolic acid and tanshinone biosynthesis in Salvia miltiorrhiza.

Abietanes Gene Expression Regulation, Plant Hydroxybenzoates Plant Proteins / genetics Plant Roots / metabolism Salvia miltiorrhiza / genetics
Hairy root Phenolic acids Salvia miltiorrhiza SmWRKY34 SmbZIP3 Tanshinones

Journal

Metabolic engineering
ISSN: 1096-7184
Titre abrégé: Metab Eng
Pays: Belgium
ID NLM: 9815657

Informations de publication

Date de publication:
09 2022
Historique:
received: 20 05 2022
revised: 26 07 2022
accepted: 01 08 2022
pubmed: 8 8 2022
medline: 14 9 2022
entrez: 7 8 2022
Statut: ppublish

Résumé

Phenolic acids and tanshinones are main bioactive compounds produced in Salvia miltiorrhiza widely used in treatment of cardiovascular diseases, which could be promoted by abscisic acid elicitation. However, the regulation mechanism remained to be elucidated. An ABA-inducible IIa WRKY transcription factor (TF) named SmWRKY34 exhibiting high homology with AtWRKY40 was isolated. SmWRKY34 exhibited a negative role on phenolic acids and tanshinones by directly regulating SmRAS and SmGGPPS. Moreover, ABA-responsive bZIP TF member named SmbZIP3 expressing significantly in SmWRKY34 transcriptome was screened. SmWRKY34 showed a negative regulatory role on SmbZIP3. SmbZIP3 acted as a positive regulator in the biosynthesis of phenolic acids and tanshinones by targeting SmTAT and two tanshinone-promoting TFs SmERF128 and SmMYB9b. Taken together, we identify a new module WRKY34-bZIP3 involved in ABA signaling that manipulates phenolic acid and tanshinone accumulation, shedding new insights in metabolic engineering application in S. miltiorrhiza.

Identifiants

DOI: 10.1016/j.ymben.2022.08.002 PMID: 35934177
pubmed: 35934177
pii: S1096-7176(22)00097-0
doi: 10.1016/j.ymben.2022.08.002
pii:
doi:

Substances chimiques

Abietanes 0
Hydroxybenzoates 0
Plant Proteins 0
tanshinone 03UUH3J385
phenolic acid I3P9R8317T

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

182-191

Informations de copyright

Copyright © 2022 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare that they have no competing interests.

Auteurs

Min Shi (M)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China; State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.

Ruiyan Zhu (R)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Yi Zhang (Y)

Institute of Plant Biotechnology, School of Life Sciences, Shanghai Normal University, Shanghai, 200234, PR China.

Siwei Zhang (S)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Tingyao Liu (T)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Kunlun Li (K)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Shucan Liu (S)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Leran Wang (L)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Yao Wang (Y)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China; Institute of Plant Biotechnology, School of Life Sciences, Shanghai Normal University, Shanghai, 200234, PR China.

Wei Zhou (W)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China.

Qiang Hua (Q)

State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, PR China.

Guoyin Kai (G)

Laboratory of Medicinal Plant Biotechnology, School of Pharmaceutical Sciences, Zhejiang Chinese Medical University, Hangzhou, Zhejiang, 310053, PR China. Electronic address: kaiguoyin@zcmu.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Composés polycycliques › Hydrocarbures aromatiques polycycliques › Phénanthrènes › Diterpènes de type abiétane › A novel WRKY34-bZIP3 module regulates phenolic...
questionsmedicales.fr › Phénomènes génétiques › Régulation de l'expression des gènes › Régulation de l'expression des gènes végétaux › A novel WRKY34-bZIP3 module regulates phenolic...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures cycliques › Hydrocarbures aromatiques › Dérivés du benzène › Phénols › Hydroxybenzoates › A novel WRKY34-bZIP3 module regulates phenolic...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines végétales › A novel WRKY34-bZIP3 module regulates phenolic...
questionsmedicales.fr › Structures de plante › Racines de plante › A novel WRKY34-bZIP3 module regulates phenolic...
questionsmedicales.fr › Eucaryotes › Viridiplantae › Streptophyta › Embryophyta › Tracheobionta › Magnoliopsida › Lamiales › Lamiaceae › Salvia › Salvia miltiorrhiza › A novel WRKY34-bZIP3 module regulates phenolic...