Ethanolic extract of Coleus aromaticus leaves impedes the proliferation and instigates apoptotic cell death in liver cancer HepG2 cells through repressing JAK/STAT cascade.
Antioxidants
Apoptosis
Carcinoma, Hepatocellular
/ drug therapy
Carotenoids
Caspase 8
Cell Proliferation
Chlorogenic Acid
Coleus
Dietary Fiber
Ethanol
Hep G2 Cells
Humans
Janus Kinases
/ metabolism
Liver Neoplasms
/ drug therapy
Myeloid Cell Leukemia Sequence 1 Protein
Phenols
Plant Extracts
/ pharmacology
Reactive Oxygen Species
STAT Transcription Factors
/ metabolism
Thymol
Coleus aromaticus
HepG2 cells
anticancer
apoptosis
liver cancer
Journal
Journal of food biochemistry
ISSN: 1745-4514
Titre abrégé: J Food Biochem
Pays: United States
ID NLM: 7706045
Informations de publication
Date de publication:
10 2022
10 2022
Historique:
revised:
14
06
2022
received:
27
04
2022
accepted:
21
06
2022
pubmed:
11
8
2022
medline:
13
10
2022
entrez:
10
8
2022
Statut:
ppublish
Résumé
Liver cancer or hepatocellular carcinoma (HCC) has become a leading cause for cancer burden across the globe, and incidences have tripled since the last two decades. Poor diagnosis of primary liver cancer and limited treatment strategies aggravate the challenges. Researchers globally have shown a steep inclination toward the exploration of plant-based compounds for their nutraceutical and anticancer potential to fit into the role of novel chemotherapeutics. Coleus aromaticus is a well-known culinary herb that earlier has been reported for several medicinal attributes. The current investigation deals with exploring the anticancer potential of ethanolic leaf extract of C. aromaticus (CoL-EtOH) against hepatocellular carcinoma HepG2 cell line. The observations made it evident that CoL-EtOH extract impeded the viability of HepG2 at 400 μg/ml (p < .01). Additionally, the extract also succeeded in escalating ROS production (p < .01) which aided dissipation of mitochondrial membrane potential and disruption of nuclear morphology. CoL-EtOH further activated caspase-8, -9, and -3 which was reaffirmed by increase in apoptosis at 400 μg/ml (p < .01). Moreover, post treatment with CaLEt-OH extract significantly reduced the expression of JAK-1 & STAT-3 genes (p < .01) along with regulated expression of Mcl1, Bcl-2, cyclinD1, p21, and p27 within HepG2 cells. This evidence portrays the promising anticancer potential of CoL-EtOH projecting it as a novel chemotherapeutic agent against HCC. PRACTICAL APPLICATIONS: The herb Coleus aromaticus belonging to Lamiaceae family and Coleus genus is known by various names in different regions of the world and several language-specific vernacular names. The herb has been used in therapeutic and medicinal applications as well as in culinary preparations. Various attributes of the nutritional strength and functional characteristics of the leaves in terms of carotenoids, minerals, phenols, dietary fiber, and antioxidant activity have been reported by several researchers. Carvacrol and thymol are majorly found in the plant, while chlorogenic acid and rosmarinic acid etc. as the phenolic components. The herb has been used in therapeutic and medicinal implications as well as in culinary preparations.
Substances chimiques
Antioxidants
0
Dietary Fiber
0
Myeloid Cell Leukemia Sequence 1 Protein
0
Phenols
0
Plant Extracts
0
Reactive Oxygen Species
0
STAT Transcription Factors
0
Chlorogenic Acid
318ADP12RI
Carotenoids
36-88-4
Thymol
3J50XA376E
Ethanol
3K9958V90M
Janus Kinases
EC 2.7.10.2
Caspase 8
EC 3.4.22.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14368Informations de copyright
© 2022 Wiley Periodicals LLC.
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