RecA and RecB: probing complexes of DNA repair proteins with mitomycin C in live
DNA damage
mitomycin C
recombination
repair
single-molecule tracking
super-resolution microscopy
Journal
Journal of the Royal Society, Interface
ISSN: 1742-5662
Titre abrégé: J R Soc Interface
Pays: England
ID NLM: 101217269
Informations de publication
Date de publication:
08 2022
08 2022
Historique:
entrez:
10
8
2022
pubmed:
11
8
2022
medline:
12
8
2022
Statut:
ppublish
Résumé
The RecA protein and RecBCD complex are key bacterial components for the maintenance and repair of DNA. RecBCD is a helicase-nuclease that uses homologous recombination to resolve double-stranded DNA breaks. It also facilitates coating of single-stranded DNA with RecA to form RecA filaments, a vital step in the double-stranded break DNA repair pathway. However, questions remain about the mechanistic roles of RecA and RecBCD in live cells. Here, we use millisecond super-resolved fluorescence microscopy to pinpoint the spatial localization of fluorescent reporters of RecA or RecB at physiological levels of expression in individual live
Identifiants
pubmed: 35946163
doi: 10.1098/rsif.2022.0437
pmc: PMC9363994
doi:
Substances chimiques
DNA, Bacterial
0
DNA, Single-Stranded
0
Escherichia coli Proteins
0
Mitomycin
50SG953SK6
DNA
9007-49-2
Exodeoxyribonuclease V
EC 3.1.11.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
20220437Subventions
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/P000746/1
Pays : United Kingdom
Organisme : Biotechnology and Biological Sciences Research Council
ID : BB/N006453/1
Pays : United Kingdom
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