Endophytic bacteria from in vitro culture of Leucojum aestivum L. a new source of galanthamine and elicitor of alkaloid biosynthesis.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
11 08 2022
11 08 2022
Historique:
received:
16
03
2022
accepted:
03
08
2022
entrez:
11
8
2022
pubmed:
12
8
2022
medline:
16
8
2022
Statut:
epublish
Résumé
Leucojum aestivum is known for its ability to biosynthesize alkaloids with therapeutic properties, among which galanthamine used for the treatment of Alzheimer's disease. New sources of this alkaloid are still being explored. In this study, a novel strain PLV of endophytic bacterium Paenibacillus lautus was isolated from in vitro L. aestivum plants. We report the whole genome sequence of that strain and its capacity to produce alkaloids and growth regulators. The effect of elicitation with autoclaved bacteria on the production of alkaloids was examined. Ten alkaloids were identified in bacteria extracts: galanthamine, lycorine, ismine, lycoramine, haemanthamine, tazettine, galanthine, homolycorine, 1,2-dihydrochlidanthine, and hippeastrine. The mean contents of galanthamine and lycorine were 37.51 µg/g of dry weight (DW) and 129.93 µg/g of DW, respectively. Moreover, isolated P. lautus strain synthesized: indole-3-acetic acid, t-zeatin, c-zeatin, kinetin, gibberellin A
Identifiants
pubmed: 35953692
doi: 10.1038/s41598-022-17992-5
pii: 10.1038/s41598-022-17992-5
pmc: PMC9371375
doi:
Substances chimiques
Alkaloids
0
Amaryllidaceae Alkaloids
0
Galantamine
0D3Q044KCA
Zeatin
7I6OOJ9GR6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
13700Informations de copyright
© 2022. The Author(s).
Références
J Biotechnol. 2010 Jul 20;148(2-3):99-104
pubmed: 20576504
J Ethnopharmacol. 2004 Jun;92(2-3):147-62
pubmed: 15137996
Molecules. 2020 Oct 23;25(21):
pubmed: 33113950
Appl Microbiol Biotechnol. 2015 Sep;99(18):7651-62
pubmed: 25958995
PeerJ. 2020 Mar 16;8:e8688
pubmed: 32211230
Bioinformatics. 2013 Apr 15;29(8):1072-5
pubmed: 23422339
Plants (Basel). 2021 Jan 01;10(1):
pubmed: 33401438
J Biotechnol. 2009 Jan 1;139(1):84-8
pubmed: 18983879
Planta. 2016 May;243(5):1097-114
pubmed: 26794966
Plant Physiol Biochem. 2016 Sep;106:236-43
pubmed: 27182958
J Biol Chem. 1969 Nov 25;244(22):6049-55
pubmed: 5389100
J Nat Prod. 2009 Jan;72(1):142-7
pubmed: 19117485
Nat Commun. 2019 May 16;10(1):2182
pubmed: 31097708
J Bacteriol. 1951 Sep;62(3):293-300
pubmed: 14888646
Sci Rep. 2018 Apr 3;8(1):5450
pubmed: 29615668
Bioinformation. 2010 Nov 01;5(5):191-7
pubmed: 21364796
Chirality. 2017 Sep;29(9):486-499
pubmed: 28649696
Nucleic Acids Res. 2016 Aug 19;44(14):6614-24
pubmed: 27342282
Curr Opin Plant Biol. 2011 Aug;14(4):435-43
pubmed: 21536480
Sci China Life Sci. 2017 Apr;60(4):417-428
pubmed: 28251459
Molecules. 2017 May 23;22(6):
pubmed: 28545250
Microorganisms. 2019 Dec 22;8(1):
pubmed: 31877906
Anal Biochem. 1976 May 7;72:248-54
pubmed: 942051
N Biotechnol. 2019 Jan 25;48:1-11
pubmed: 29017819
BMC Microbiol. 2012 Jan 12;12:3
pubmed: 22235902
Mol Biol Evol. 2015 Oct;32(10):2798-800
pubmed: 26130081
Environ Microbiol. 2020 Apr;22(4):1421-1434
pubmed: 32090436
Molecules. 2020 Oct 13;25(20):
pubmed: 33066212
Phytomedicine. 2021 Jun;86:153440
pubmed: 33376043
Microbiol Res. 2020 Oct;239:126501
pubmed: 32585579
Int J Mol Sci. 2021 Feb 10;22(4):
pubmed: 33578992
Plant Physiol Biochem. 2017 Sep;118:494-509
pubmed: 28756347
Int J Microbiol. 2020 Dec 10;2020:8839490
pubmed: 33488726
Bioinformatics. 2015 Aug 1;31(15):2443-51
pubmed: 25810435
J Comput Biol. 2012 May;19(5):455-77
pubmed: 22506599
Appl Microbiol Biotechnol. 2007 Oct;76(5):1145-52
pubmed: 17657487
World J Microbiol Biotechnol. 1991 Jul;7(4):452-69
pubmed: 24425131
PLoS One. 2014 Nov 19;9(11):e112963
pubmed: 25409509
Sci Rep. 2016 Dec 06;6:38284
pubmed: 27922057
Appl Microbiol Biotechnol. 2011 Apr;90(2):461-6
pubmed: 21222119
Methods Enzymol. 1984;105:121-6
pubmed: 6727660