Rational engineering of a synthetic insect-bacterial mutualism.
bacteria
genome engineering
insect
mutualism
symbiosis
synthetic biology
Journal
Current biology : CB
ISSN: 1879-0445
Titre abrégé: Curr Biol
Pays: England
ID NLM: 9107782
Informations de publication
Date de publication:
26 09 2022
26 09 2022
Historique:
received:
11
04
2022
revised:
25
05
2022
accepted:
14
07
2022
pubmed:
14
8
2022
medline:
30
9
2022
entrez:
13
8
2022
Statut:
ppublish
Résumé
Many insects maintain mutualistic associations with bacterial endosymbionts, but little is known about how they originate in nature. In this study, we describe the establishment and manipulation of a synthetic insect-bacterial symbiosis in a weevil host. Following egg injection, the nascent symbiont colonized many tissues, including prototypical somatic and germinal bacteriomes, yielding maternal transmission over many generations. We then engineered the nascent symbiont to overproduce the aromatic amino acids tyrosine and phenylalanine, which facilitate weevil cuticle strengthening and accelerated larval development, replicating the function of mutualistic symbionts that are widely distributed among weevils and other beetles in nature. Our work provides empirical support for the notion that mutualistic symbioses can be initiated in insects by the acquisition of environmental bacteria. It also shows that certain bacterial genera, including the Sodalis spp. used in our study, are predisposed to develop these associations due to their ability to maintain benign infections and undergo vertical transmission in diverse insect hosts, facilitating the partner-fidelity feedback that is critical for the evolution of obligate mutualism. These experimental advances provide a new platform for laboratory studies focusing on the molecular mechanisms and evolutionary processes underlying insect-bacterial symbiosis.
Identifiants
pubmed: 35963240
pii: S0960-9822(22)01163-0
doi: 10.1016/j.cub.2022.07.036
pmc: PMC10080585
mid: NIHMS1884393
pii:
doi:
Substances chimiques
Amino Acids, Aromatic
0
Tyrosine
42HK56048U
Phenylalanine
47E5O17Y3R
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3925-3938.e6Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM128804
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM136389
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM142950
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2022 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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