Cell Synchronization Techniques for Studying Mitosis.

Cell Cycle G1 Phase Microtubules Mitosis Nocodazole / pharmacology
Cell cycle synchronization Double thymidine block MG132 Mitotic arrest Nocodazole STLC Timelapse imaging

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 31 8 2022
pubmed: 1 9 2022
medline: 9 9 2022
Statut: ppublish

Résumé

Cell synchronization allows the examination of cell cycle progression. Nocodazole and other microtubule poisons have been used extensively to interfere with microtubule function and arrest cells in mitosis. Since microtubules are important for many cellular functions, alternative cell cycle synchronization techniques independent of microtubule inhibition are also used for synchronizing cells in mitosis. Here we describe using nocodazole, STLC, and combining thymidine block with MG132 to synchronize cells in mitosis. These inhibitors are reversible and mitotic cells can be released into the G1 phase synchronously. These techniques can be applied to both Western blot and timelapse imaging to study mitotic progression.

Identifiants

DOI: 10.1007/978-1-0716-2736-5_6 PMID: 36045199
pubmed: 36045199
doi: 10.1007/978-1-0716-2736-5_6
doi:

Substances chimiques

Nocodazole SH1WY3R615

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

73-86

Subventions

Organisme : CIHR
ID : PJT-159585
Pays : Canada

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

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Auteurs

Joanne D Hadfield (JD)

Department of Oncology, University of Alberta, Edmonton, AB, Canada.
Experimental Oncology, Cross Cancer Institute, Edmonton, AB, Canada.
Cancer Research Institute of Northern Alberta, University of Alberta, Edmonton, AB, Canada.

Sargun Sokhi (S)

Department of Oncology, University of Alberta, Edmonton, AB, Canada.
Cancer Research Institute of Northern Alberta, University of Alberta, Edmonton, AB, Canada.

Gordon K Chan (GK)

Department of Oncology, University of Alberta, Edmonton, AB, Canada. gkc@ualberta.ca.
Experimental Oncology, Cross Cancer Institute, Edmonton, AB, Canada. gkc@ualberta.ca.
Cancer Research Institute of Northern Alberta, University of Alberta, Edmonton, AB, Canada. gkc@ualberta.ca.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Cell Synchronization Techniques for Studying Mitosis.
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Cycle cellulaire › Interphase › Phase G1 › Cell Synchronization Techniques for Studying Mitosis.
questionsmedicales.fr › Cellules › Structures cellulaires › Espace intracellulaire › Cytoplasme › Structures cytoplasmiques › Cytosquelette › Microtubules › Cell Synchronization Techniques for Studying Mitosis.
questionsmedicales.fr › Phénomènes génétiques › Division du noyau cellulaire › Mitose › Cell Synchronization Techniques for Studying Mitosis.
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Benzimidazoles › Nocodazole › Cell Synchronization Techniques for Studying Mitosis.