Fusion of Formate Dehydrogenase and Alanine Dehydrogenase as an Amino Donor Regenerating System Coupled to Transaminases.
alanine dehydrogenase
biocatalysis
formate dehydrogenase
fusion proteins
transaminases
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
04 11 2022
04 11 2022
Historique:
revised:
05
09
2022
received:
27
07
2022
pubmed:
7
9
2022
medline:
9
11
2022
entrez:
6
9
2022
Statut:
ppublish
Résumé
Fusion enzymes are attractive tools for facilitating the assembly of biocatalytic cascades for chemical synthesis. This approach can offer great advantages for cooperative redox cascades that need the constant supply of a donor molecule. In this work, we have developed a self-sufficient bifunctional enzyme that can be coupled to transaminase-catalyzed reactions for the efficient recycling of the amino donor (L-alanine). By genetic fusion of an alanine dehydrogenase (AlaDH) and a formate dehydrogenase (FDH), a redox-complementary system was applied to recycle the amino donor and the cofactor (NADH), respectively. AlaDH and FDH were assembled in both combinations (FDH-AlaDH and AlaDH-FDH), with a 2.5-fold higher enzymatic activity of the latter system. Then, AlaDH-FDH was coupled to two different S-selective transaminases for the synthesis of vanillyl amine (10 mM) reaching up to 99 % conversion in 24 h in both cases. Finally, the multienzyme system was reused for at least 3 consecutive cycles when implemented in dialysis-assisted biotransformations.
Identifiants
pubmed: 36066500
doi: 10.1002/cbic.202200428
pmc: PMC9828552
doi:
Substances chimiques
Formate Dehydrogenases
EC 1.17.1.9
Alanine Dehydrogenase
EC 1.4.1.1
Transaminases
EC 2.6.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202200428Informations de copyright
© 2022 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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