Generation of two induced pluripotent stem cell (iPSC) lines from patients with Duchenne muscular dystrophy (IGIBi006-A and IGIBi008-A) carrying exonic deletions in the dystrophin gene.


Journal

Stem cell research
ISSN: 1876-7753
Titre abrégé: Stem Cell Res
Pays: England
ID NLM: 101316957

Informations de publication

Date de publication:
10 2022
Historique:
received: 15 09 2022
revised: 19 09 2022
accepted: 24 09 2022
pubmed: 4 10 2022
medline: 26 10 2022
entrez: 3 10 2022
Statut: ppublish

Résumé

Duchenne muscular dystrophy (DMD) is an X-linked recessive disorder with defect in dystrophin gene that shows features of degeneration of muscle tissue at an early age. Here, we describe iPSC lines generated from LCL of two patients of Indian origin carrying 46-48 and 49-50 exons deletions in DMD. The resulting iPSC lines IGIBi006-A and IGIBi008-A showed all the characteristic features of pluripotency, differentiated into cells of three germ layers in vitro and have no major genetic alterations due to reprogramming process. These lines can serve as a useful cell model for studying disease pathogenesis and will aid in precision therapy.

Identifiants

pubmed: 36191544
pii: S1873-5061(22)00276-8
doi: 10.1016/j.scr.2022.102927
pii:
doi:

Substances chimiques

Dystrophin 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

102927

Informations de copyright

Copyright © 2022 CSIR-Institute of Genomics and Integrative Biology. Published by Elsevier B.V. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

Auteurs

Istaq Ahmad (I)

Department of Neurology, Neurosciences Center, All India Institutes of Medical Sciences (AIIMS), New Delhi 110029, India.

Divya Goel (D)

Division of Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology (IGIB), New Delhi 110007, India; Department of Pharmacology, School of Pharmaceutical Education & Research (SPER), Jamia Hamdard, New Delhi 110062, India.

Anindita Ghosh (A)

Division of Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology (IGIB), New Delhi 110007, India.

Himanshi Kapoor (H)

Division of Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology (IGIB), New Delhi 110007, India.

Deepak Kumar (D)

Division of Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology (IGIB), New Delhi 110007, India; Department of Zoology, University of Allahabad, Prayagraj, Uttar Pradesh 211002, India.

Keerthi Ramesh (K)

Dystrophy Annihilation Research Trust (DART), Bangalore 560094, India.

Berty Ashley (B)

Dystrophy Annihilation Research Trust (DART), Bangalore 560094, India.

K Deepika (K)

Dystrophy Annihilation Research Trust (DART), Bangalore 560094, India.

Arun Shastry (A)

Dystrophy Annihilation Research Trust (DART), Bangalore 560094, India.

Mohammed Faruq (M)

Division of Genomics and Molecular Medicine, CSIR - Institute of Genomics and Integrative Biology (IGIB), New Delhi 110007, India; Academy of Scientific and Innovative Research (AcSIR), Ghaziabad 201002, India; Division of Investigations of Human Pathology by Application Genomics-Exomics & Stem Cells (StemAppGenE, SAGEx) CSIR - IGIB, New Delhi, 110007, India. Electronic address: faruq.mohd@igib.res.in.

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