Early Nitrogenase Ancestors Encompassed Novel Active Site Diversity.
Nif
ancestral sequence reconstruction
early life
nitrogenase
Journal
Molecular biology and evolution
ISSN: 1537-1719
Titre abrégé: Mol Biol Evol
Pays: United States
ID NLM: 8501455
Informations de publication
Date de publication:
03 11 2022
03 11 2022
Historique:
pubmed:
20
10
2022
medline:
10
11
2022
entrez:
19
10
2022
Statut:
ppublish
Résumé
Ancestral sequence reconstruction (ASR) infers predicted ancestral states for sites within sequences and can constrain the functions and properties of ancestors of extant protein families. Here, we compare the likely sequences of inferred nitrogenase ancestors to extant nitrogenase sequence diversity. We show that the most-likely combinations of ancestral states for key substrate channel residues are not represented in extant sequence space, and rarely found within a more broadly defined physiochemical space-supporting that the earliest ancestors of extant nitrogenases likely had alternative substrate channel composition. These differences may indicate differing environmental selection pressures acting on nitrogenase substrate specificity in ancient environments. These results highlight ASR's potential as an in silico tool for developing hypotheses about ancestral enzyme functions, as well as improving hypothesis testing through more targeted in vitro and in vivo experiments.
Identifiants
pubmed: 36260513
pii: 6763667
doi: 10.1093/molbev/msac226
pmc: PMC9641968
pii:
doi:
Substances chimiques
Nitrogenase
EC 1.18.6.1
Proteins
0
Banques de données
figshare
['10.6084/m9.figshare.c.6039527']
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2022. Published by Oxford University Press on behalf of Society for Molecular Biology and Evolution.
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